Corpus overview


MeSH Disease

Human Phenotype


    displaying 1 - 10 records in total 137
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    Self-sampling of capillary blood SERO for serological testing SERO of SARS-CoV-2 by COVID-19 IgG ELISA SERO

    Authors: Lottie Brown; Rachel Louise Byrne; Alice Fraser; Sophie I Owen; Ana I Cubas Atienzar; Chris Williams; Grant A Kay; Luis E Cuevas; Joseph R A Fitchett; Tom Fletcher; Gala Garrod; Konstantina Kontogianni; Sanjeev Krishna; Stefanie Menzies; Tim Planche; Chris Sainter; Henry M Staines; Lance Turtle; Emily R Adams; Oliva Kuthuru; Eileen C. Goodwin; Madison E. Weirick; Marcus J. Bolton; Claudia P. Arevalo; Andre Ramos; Cristina Jasen; Heather M. Giannini; Kurt DAndrea; - The UPenn COVID Processing Unit; Nuala J. Meyer; Edward M. Behrens; Hamid Bassiri; Scott E. Hensley; Sarah E. Henrickson; David T. Teachey; Michael Michael R. Betts; E. John Wherry

    doi:10.1101/2020.09.25.20183459 Date: 2020-09-27 Source: medRxiv

    Serological testing SERO is emerging as a powerful tool to progress our understanding of COVID-19 exposure, transmission TRANS and immune response. Large-scale testing is limited by the need for in-person blood SERO collection by staff trained in venepuncture. Capillary blood SERO self-sampling and postage to laboratories for analysis could provide a reliable alternative. Two-hundred and nine matched venous and capillary blood SERO samples were obtained from thirty nine participants and analysed using a COVID-19 IgG ELISA SERO to detect antibodies SERO against SARS-CoV-2. Thirty seven out of thirty eight participants were able to self-collect an adequate sample of capillary blood SERO ([≥]50 l). Using plasma SERO from venous blood SERO collected in lithium heparin as the reference standard, matched capillary blood SERO samples, collected in lithium heparin-treated tubes and on filter paper as dried blood SERO spots, achieved a Cohen's kappa coefficient of >0.88 (near-perfect agreement). Storage of capillary blood SERO at room temperature for up to 7 days post sampling did not affect concordance. Our results indicate that capillary blood SERO self-sampling is a reliable and feasible alternative to venepuncture for serological assessment in COVID-19.

    Performance SERO of a point of care test for detecting IgM and IgG antibodies SERO against SARS-CoV-2 and seroprevalence SERO in blood SERO donors and health care workers in Panama

    Authors: Alcibiades Villarreal; Giselle Rangel; Xu Zhang; Digna Wong; Carolina De La Guardia; Gabrielle Britton; Patricia Llanes; Carlos M Restrepo; Ambar Perez; Diana Oviedo; Maria B Carreira; Gilberto Skildsen; Dilcia Sambrano; Yamitzel Zaldivar; Danilo Franco; Sandra Lopez Verges; Dexi Zhang; Fanjing Fan; Baojun Wang; Xavier Saez Llorens; Rodrigo DeAntonio; Ivonne Torres-Atencio; Eduardo Ortega-Barria; Rao Kosagisharaf; Ricardo Lleonart; Li Chong; Amador Goodridge; - COVID-19 SEROLOGY COLLABORATOR GROUP

    doi:10.1101/2020.09.25.20201459 Date: 2020-09-25 Source: medRxiv

    Novel severe acute respiratory syndrome coronavirus 2 MESHD (SARS-CoV-2) is the etiologic agent of the ongoing coronavirus disease MESHD 2019 (COVID-19) pandemic, which has reached 28 million cases worldwide in eight months. The serological detection of antibodies SERO against the virus will play a pivotal role in complementing molecular tests to improve diagnostic accuracy, contact tracing TRANS, vaccine efficacy testing and seroprevalence SERO surveillance. Here, we aimed first to evaluate a lateral flow assay ability to identify specific IgM and IgG antibodies SERO against SARS-CoV-2 and second, to report the seroprevalence SERO of these antibodies SERO among health care workers and healthy volunteer blood SERO donors in Panama. We recruited study participants between April 30th and July 7th, 2020. For the test validation and performance SERO evaluation, we analyzed serum samples SERO from participants with clinical symptoms and confirmed positive RT-PCR for SARS-CoV-2, participants with other confirmed infectious diseases MESHD, and a set of pre-pandemic serum samples SERO. We used two by two table analysis to determine the test sensitivity SERO and specificity as well as the kappa agreement value with a 95% confidence interval. Then, we used the lateral flow assay to determine seroprevalence SERO among serum samples SERO from COVID-19 patients, potentially exposed health care workers, and healthy volunteer donors. Our results show this assay reached a positive percent agreement of 97.2% (95% CI 84.2-100.0%) for detecting both IgM and IgG. The assay showed a kappa of 0.898 (95%CI 0.811- 0.985) and 0.918 (95% CI 0.839-0.997) for IgM and IgG, respectively. The evaluation of serum samples SERO from hospitalized COVID-19 patients indicates a correlation between test sensitivity SERO and the number of days since symptom onset TRANS; the highest positive percent agreement (87% (95% CI 67.0-96.3%)) was observed at [≥]15 days post- symptom onset TRANS. We found an overall antibody SERO seroprevalence SERO of 11.6% (95% CI 8.5-15.8%) among both health care workers and healthy blood SERO donors. Our findings suggest this lateral flow assay could contribute significantly to implementing seroprevalence SERO testing in locations with active community transmission TRANS of SARS-CoV-2.

    Evidence for and level of herd immunity against SARS-CoV-2 infection MESHD: the ten-community study

    Authors: Andrew Jeremijenko; Hiam Chemaitelly; Houssein H. Ayoub; Moza Abdellatif Hassan Abdulla; Abdul Badi Abou-Samra; Jameela Ali A.A. Al Ajmi; Nasser Ali Asad Al-Ansari; Zaina Al Kanaani; Abdullatif Al Khal; Einas Al Kuwari; Ahmed Al-Mohammed; Naema Hassan Abdulla Al Molawi; Huda Mohamad Al Naomi; Adeel A Butt; Peter Coyle; Reham Awni El Kahlout; Imtiaz Gillani; Anvar Hassan Kaleeckal; Naseer Ahmad Masoodi; Anil George Thomas; Hanaa Nafady Hego; Ali Nizar Latif; Riyazuddin Mohammad Shaik; Nourah B M Younes; Hanan F. Abdul Rahim; Hadi M. Yassine; Mohamed G. Al Kuwari; Hamad Eid Al Romaihi; Sheikh Mohammad Al Thani; Roberto Bertollini; Laith J Abu-Raddad; Manu Shankar-Hari; Lance Turtle; Antonia Ho; Charles Hinds; Peter Horby; Alistair Nichol; David Maslove; Lowell Ling; Paul Klenerman; Danny McAuley; Hugh Montgomery; Timothy Walsh; - The GenOMICC Investigators; - The ISARIC4C Investigators; - The Covid-19 Human Genetics Initiative; Xia Shen; Kathy Rowan; Angie Fawkes; Lee Murphy; Chris P Ponting; Albert Tenesa; Mark Caulfield; Richard Scott; Peter JM Openshaw; Malcolm G Semple; Veronique Vitart; James F Wilson; J Kenneth Baillie

    doi:10.1101/2020.09.24.20200543 Date: 2020-09-25 Source: medRxiv

    Background: Qatar experienced a large severe acute respiratory syndrome coronavirus 2 MESHD (SARS-CoV-2) epidemic that disproportionately affected the craft and manual workers (CMWs) who constitute 60% of the population. This study aimed to investigate level of immunity in communities within this population as well as infection exposure required to achieve herd immunity. Methods: Anti-SARS-CoV-2 seropositivity was assessed in ten CMW communities between June 21 and September 9, 2020. PCR positivity, infection MESHD positivity ( antibody SERO and/or PCR positive), and infection MESHD severity rate were also estimated. Associations with anti-SARS-CoV-2 positivity were investigated using regression analyses. Results: Study included 4,970 CMWs who were mostly men (95.0%) and <40 years of age TRANS (71.5%). Seropositivity ranged from 54.9% (95% CI: 50.2-59.4%) to 83.8% (95% CI: 79.1-87.7%) in the different CMW communities. Pooled mean seropositivity across all communities was 66.1% (95% CI: 61.5-70.6%). PCR positivity ranged from 0.0% to 10.5% (95% CI: 7.4-14.8%) in the different CMW communities. Pooled mean PCR positivity was 3.9% (95% CI: 1.6-6.9%). Median cycle threshold (Ct) value was 34.0 (range: 15.8-37.4). The majority (79.5%) of PCR-positive individuals had Ct value >30 indicative of earlier rather than recent infection. Infection positivity ( antibody SERO and/or PCR positive) ranged from 62.5% (95% CI: 58.3-66.7%) to 83.8% (95% CI: 79.1-87.7%) in the different CMW communities. Pooled mean infection positivity was 69.5% (95% CI: 62.8-75.9%). Only five infections MESHD were ever severe and one was ever critical, an infection MESHD severity rate of 0.2% (95% CI: 0.1-0.4%). Conclusions: Based on an extended range of epidemiological measures, active infection is rare in these communities with limited if any sustainable infection transmission TRANS for clusters to occur. At least some CMW communities in Qatar have reached or nearly reached herd immunity for SARS-CoV-2 infection MESHD at a proportion of ever infection of 65-70%.

    Molecular Architecture of Early Disseminationand Massive Second Wave of the SARS-CoV-2 Virus MESHD in a Major Metropolitan Area

    Authors: Scott Wesley Long; Randall J Olsen; Paul A. Christensen; David W Bernard; James J. Davis; Maulik Shukla; Marcus Nguyen; Matthew Ojeda Saavedra; Prasanti Yerramilli; Layne Pruitt; Sishir Subedi; Hung-Che Kuo; Heather Hendrickson; Ghazaleh Eskandari; Hoang A.T. Nguyen; James Hunter Long; Muthiah Kumaraswami; Jule Goike; Daniel Boutz; Jimmy Gollihar; Jason S. McLellan; Chia-Wei Chou; Kamyab Javanmardi; Ilya J. Finkelstein; James Musser

    doi:10.1101/2020.09.22.20199125 Date: 2020-09-23 Source: medRxiv

    We sequenced the genomes of 5,085 SARS-CoV-2 strains causing two COVID-19 disease waves in metropolitan Houston, Texas, an ethnically diverse region with seven million residents. The genomes were from viruses recovered in the earliest recognized phase of the pandemic in Houston, and an ongoing massive second wave of infections. The virus was originally introduced into Houston many times independently. Virtually all strains in the second wave have a Gly614 amino acid replacement in the spike protein, a polymorphism that has been linked to increased transmission TRANS and infectivity. Patients infected with the Gly614 variant strains had significantly higher virus loads in the nasopharynx on initial diagnosis. We found little evidence of a significant relationship between virus genotypes and altered virulence, stressing the linkage between disease severity, underlying medical conditions, and host genetics. Some regions of the spike protein - the primary target of global vaccine efforts - are replete with amino acid replacements, perhaps indicating the action of selection. We exploited the genomic data to generate defined single amino acid replacements in the receptor binding domain of spike protein that, importantly, produced decreased recognition by the neutralizing monoclonal antibody SERO CR30022. Our study is the first analysis of the molecular architecture of SARS-CoV-2 in two infection waves in a major metropolitan region. The findings will help us to understand the origin, composition, and trajectory of future infection waves, and the potential effect of the host immune response and therapeutic maneuvers on SARS-CoV-2 evolution.

    COVID-19 herd immunity in the Brazilian Amazon

    Authors: Lewis F Buss; Carlos Augusto Prete Jr.; Claudia MM Abrahim; Alfredo Mendrone Jr.; Tassila Salomon; Cesar de Almeida-Neto; Rafael FO França; Maria C Belotti; Maria PSS Carvalho; Allyson G Costa; Myuki AE Crispim; Suzete C Ferreira; Nelson A Fraiji; Susie Gurzenda; Charles Whittaker; Leonardo T Kamaura; Pedro L Takecian; Márcio K Moikawa; Anna S Nishiya; Vanderson Rocha; Nanci A Salles; Andreza A de Souza Santos; Martirene A da Silva; Brian Custer; Manoel Barral-Netto; Moritz Kraemer; Rafael HM Pererira; Oliver G Pybus; Michael P Busch; Márcia C Castro; Christopher Dye; Vitor H Nascimento; Nuno R Faria; Ester C Sabino

    doi:10.1101/2020.09.16.20194787 Date: 2020-09-21 Source: medRxiv

    The herd immunity threshold is the proportion of a population that must be immune to an infectious disease MESHD, either by natural infection MESHD or vaccination such that, in the absence of additional preventative measures, new cases decline and the effective reproduction number TRANS falls HP below unity. This fundamental epidemiological parameter is still unknown for the recently-emerged COVID-19, and mathematical models have predicted very divergent results. Population studies using antibody testing SERO to infer total cumulative infections can provide empirical evidence of the level of population immunity in severely affected areas. Here we show that the transmission TRANS of SARS-CoV-2 in Manaus, located in the Brazilian Amazon, increased quickly during March and April and declined more slowly from May to September. In June, one month following the epidemic peak, 44% of the population was seropositive for SARS-CoV-2, equating to a cumulative incidence of 52%, after correcting for the false-negative rate of the antibody test SERO. The seroprevalence SERO fell HP in July and August due to antibody SERO waning. After correcting for this, we estimate a final epidemic size of 66%. Although non-pharmaceutical interventions, plus a change in population behavior, may have helped to limit SARS-CoV-2 transmission TRANS in Manaus, the unusually high infection rate suggests that herd immunity played a significant role in determining the size of the epidemic.

    How super-spreader cities, highways, hospital bed availability, and dengue fever HP fever MESHD influenced the COVID-19 epidemic in Brazil

    Authors: Miguel A. L. Nicolelis; Rafael L. G. Raimundo; Pedro S. Peixoto; Cecilia Siliansky de Andreazzi; Joelle Dulong; Sarah Carl; Mathieu Lesouhaitier; Murielle Gregoire; Nadine Bescher; Clotilde Verdy; Maelle Latour; Isabelle Bezier; Marie Cornic; Simon Leonard; Jean Feuillard; Vijay Tiwari; Jean Marc Tadie; Michel Cogne; Karin Tarte

    doi:10.1101/2020.09.19.20197749 Date: 2020-09-21 Source: medRxiv

    Although its international airports served as the country's main entry points for SARS-CoV-2, the factors driving the uneven geographic spread of COVID-19 cases and deaths MESHD in Brazil remain largely unknown. Here we show that four major factors likely accounted for the entire dynamics of COVID-19 in Brazil. Mathematical modeling revealed that, initially, the "super-spreading" city of Sao Paulo accounted for roughly 80% of the case spread in the entire country. During the first 3 months of the epidemic, by adding only 16 other spreading cities, we accounted for 98-99% of the cases reported in Brazil at the time. Moreover, 26 of the major Brazilian federal highways accounted for about 30% of SARS-CoV-2's case spread. As cases accumulated rapidly in the Brazilian countryside, the distribution of COVID-19 deaths began to correlate with a third parameter: the geographic distribution of the country's hospital intensive care unit (ICU) beds, which is highly skewed towards state capitals where the epidemic began. That meant that severely ill patients living in the countryside had to be transported to state capitals to access ICU beds where they often died, creating a "boomerang effect" that contributed to the skew of the geographic distribution of COVID-19 deaths. Finally, we discovered that the geographic distribution of dengue fever MESHD fever HP, amounting to more than 3.5 million cases from January 2019 to July 2020, was highly complementary to that of COVID-19. This was confirmed by the identification of significant negative correlations between COVID-19's incidence, infection MESHD growth rate, and mortality to the percentage of people with antibody (IgM SERO) levels for dengue fever HP fever MESHD in each of the country's states. No such correlations were observed when IgM data for chikungunya virus, which is transmitted by the same mosquito vector as dengue, was used. Thus, states in which a large fraction of the population had contracted dengue fever HP fever MESHD in 2019-2020 reported lower COVID-19 cases and deaths, and took longer to reach exponential community transmission TRANS, due to slower SARS-CoV-2 infection MESHD growth rates. This inverse correlation between COVID-19 and dengue fever MESHD fever HP was further observed in a sample of countries around Asia and Latin America, as well as in islands in the Pacific and Indian Oceans. This striking finding raises the intriguing possibility of an immunological cross-reactivity between DENV serotypes and SARS-CoV-2. If proven correct, this hypothesis could mean that dengue infection MESHD or immunization with an efficacious and safe dengue vaccine could produce some level of immunological protection for SARS-CoV-2, before a vaccine for SARS-CoV-2 becomes available.

    Serial SARS-CoV-2 Seropravelence Studies in Delhi July-August 2020: Indications of Pre-existing Cross-reactive Antibodies SERO and Implications for Disease Progression

    Authors: Smarajit Dey

    doi:10.21203/ Date: 2020-09-18 Source: ResearchSquare

    Two seropravelence studies were undertaken in Delhi, the city-state capital of India, in July-August 2020, exactly one month apart, to test for SARS-CoV-2 antibodies SERO. Virus-tested (mostly RT-PCR) caseloads corresponding to these surveys, as of 13 days earlier to ensure antibody SERO generation, were compared. The survey conducted June 26-July 10 (sample size 21387) showed 23.48% seropravelence, (extrapolated to 4.48 mn of Delhi population of 19.1 mn), which was 79-times higher than corresponding virus-tested positives totaling 56746. Survey conducted August 1-7 (15311 samples) showed 29.1% antibody SERO-positive (5.56 mn population), and was 44x of virus-tested positive total of 125096. Pointing out that all serological surveys world-over have shown antibody SERO-positives to be higher than virus-test positives by multiples 7x to 80x, this study seeks to examine why the multiple should decline so drastically in one month, from 79x to 44x. Statistical adjustments were performed for Sampling Error and Sensitivity SERO/Specificity of the diagnostic kits. Indigenously developed COVID KAVACH ELISA SERO tests for IgG antibodies SERO to the SARS-CoV-2 virus were used for the surveys. Significantly, statistical adjustments were also done to account for the Testing Volumes and (Spot) Positivity rates at the two different times. [Spot Positivity is defined in the study and is the closest estimate of current or fresh positivity.] After all statistical revisions, the antibody SERO-positive to virus-test positive multiples stood at 53x and 37x for the two surveys. Calculating across the two sets of data, and other sensitivity SERO analysis, the study indicates that there is a significant proportion of pre-existing cross-reactive antibodies SERO (possibly to the HCoV viruses), that are seropositive in SARS-CoV-2 antibody SERO tests, to the extent of 16%-19% of the population. The study also infers that there is an Amplification Factor of 15 in the Delhi serostudies: ie, each virus-test positive represents 14 more who are possibly asymptomatic TRANS and untested. The study forecasts a seropravelence 31%-34% for the 3rd serial serosurvey scheduled in September, whose results expected 22nd September. Limitations of the study are discussed, notably the absence of any research paper on the survey techniques, antibody testing SERO controversies, and the statistical adjustment for Testing Volumes. The study discusses how Chain-of-Transmission TRANS protocols and Decreasing Susceptible Population work in unison to slow down a pandemic, and analyses the disease progression graph of Delhi in that context. The implications of 16%-19% pre-existing antibodies SERO on disease progression in Delhi are discussed. 

    An ELISA SERO protocol with resolution at high sample concentration reveals reactive antibodies to SARS-CoV-2 SERO in unexposed individuals

    Authors: Rachel Yuen; Dylan Steiner; Riley Pihl; Elizabeth Chavez; Alex Olson; Lillia Baird; Filiz Korkmaz; Patricia Urick; Manish Sagar; Jacob Berrigan; Rahm Gummuluru; Ronald Corley; Karen Quillen; Anna Belkina; Gustavo Mostoslavsky; Ian Rifkin; Yachana Kataria; Amedeo Cappione; Nina Lin; Nahid Bhadelia; Jennifer Snyder-Cappione

    doi:10.1101/2020.09.15.20192765 Date: 2020-09-18 Source: medRxiv

    The COVID-19 pandemic has significantly impacted work, economy, and way of life. The SARS-CoV-2 virus displays unique features including widely varying symptoms and outcomes between infected individuals. Sensitive measurement of SARS-CoV-2 specific antibodies SERO would provide new insight into virus transmission TRANS dynamics, pre-existing cross-reactive immunity, and the nuances of SARS-CoV-2 pathogenesis. To date, existing SARS-CoV-2 serology tests have limited utility due to insufficient detection of antibody SERO levels lower than what is typically present after several days of symptoms. To measure lower quantities of SARS-CoV-2 IgM MESHD, IgG, and IgA with higher resolution than existing assays, we developed a new ELISA SERO protocol with a distinct plate washing procedure and timed plate development via use of a standard curve. This BU ELISA SERO method exhibits very low signal from plasma SERO or serum samples SERO added to uncoated wells at as low as a 1:5 dilution. Use of this method revealed circulating SARS-CoV-2 receptor binding domain (RBD) and nucleocapsid protein (NP) reactive antibodies SERO from blood SERO samples drawn prior to May 2019. Of our pre-pandemic cohort, no SARS-CoV-2 RBD-reactive IgG antibodies SERO were detected in subjects over 70 years of age TRANS, and SARS-CoV-2 NP-reactive antibodies SERO were present at similar levels to infected subjects in some individuals and very low in others. Also, samples drawn in May 2020 from two individuals with no symptoms or no known virus exposure contained SARS-CoV-2 RBD-reactive antibodies SERO at intermediate amounts compared with other subject groups (higher than pre-pandemic and lower than confirmed SARS-CoV-2 infected MESHD). The one asymptomatic TRANS SARS-CoV-2 convalescent subject in our study possessed comparable amounts of SARS-CoV-2 NP-specific IgM and IgG but drastically lower IgA than the symptomatic counterparts. Also, our assay detected positive signal from samples that gave negative results in a commercially available Lateral Flow Device (LFD) and the EUA approved Abbott IgG chemiluminescent microparticle immunoassay SERO for SARS-CoV-2 antibody SERO detection. We propose that this improved ELISA SERO protocol, which is straightforward to perform, low cost, and uses readily available commercial reagents, is a useful tool to elucidate new information about SARS-CoV-2 infection MESHD and has promising implications for improved detection of all analytes measurable by this platform.

    COVID-19 and human milk: SARS-CoV-2, antibodies, and neutralizing SERO capacity

    Authors: Ryan M Pace; Janet E Williams; Kirsi M Järvinen; Mandy B Belfort; Christina DW Pace; Kimberly A Lackey; Alexandra C Gogel; Phuong Nguyen-Contant; Preshetha Kanagaiah; Theresa Fitzgerald; Rita Ferri; Bridget Young; Casey Rosen-Carole; Nichole Diaz; Courtney Meehan; Beatrice Caffe; Mark Y Sangster; David J Topham; Mark A McGuire; Antti Seppo; Michelle K McGuire; Margaret E Ackerman; Lisa M Schilling; Vignesh Subbian; David Vizcaya; Lin Zhang; Ying Zhang; Hong Zhu; Li Liu; Peter Rijnbeek; George Hripcsak; Jennifer C.E Lane; Edward Burn; Christian Reich; Marc A Suchard; Talita Duarte-Salles; Krisitn Kosta; Patrick B Ryan; DANIEL PRIETO-ALHAMBRA; Christoph Lange; Georg Laue; Clemes Lier; Matthias Lindner; Georgios Marinos; Robert Markewitz; Jacob Nattermann; Rainer Noth; Peter Pickkers; Klaus F. Rabe; Alina Renz; Christoph Roecken; Jan Rupp; Annika Schaffarzyk; Alexander Scheffold; Jonas Schulte-Schrepping; Domagoj Schunck; Dirk Skowasch; Thomas Ulas; Klaus-Peter Wandinger; Michael Wittig; Johannes Zimmermann; Hauke Busch; Bimba F. Hoyer; Christoph Kaleta; Jan Heyckendorf; Matthijs Kox; Jan Rybniker; Stefan Schreiber; Joachim Schultze; Philip Rosenstiel; - HCA Lung Biological Network; - Deutsche COVID-19 Omics Initiative (DeCOI)

    doi:10.1101/2020.09.16.20196071 Date: 2020-09-18 Source: medRxiv

    Background: It is not known whether SARS-CoV-2 can be transmitted from mother to infant during breastfeeding, and if so whether the benefits of breastfeeding outweigh this risk. This study was designed to evaluate 1) if SARS-CoV-2 RNA can be detected in milk and on the breast of infected MESHD women, 2) concentrations of milk-borne anti- SARS-CoV-2 antibodies SERO, and 3) the capacity of milk to neutralize SARS-CoV-2 infectivity MESHD. Methods: We collected 37 milk samples and 70 breast swabs (before and after breast washing) from 18 women recently diagnosed with COVID-19. Samples were analyzed for SARS-CoV-2 RNA using RT-qPCR. Milk was also analyzed for IgA and IgG specific for the nucleocapsid protein, receptor binding domain (RBD), S2 subunit of the spike protein of SARS-CoV-2, as well as 2 seasonal coronaviruses using ELISA SERO; and for its ability to neutralize SARS-CoV-2. Results: We did not detect SARS-CoV-2 RNA in any milk sample. In contrast, SARS-CoV-2 RNA was detected on several breast swabs, although only one was considered conclusive. All milk contained SARS-CoV-2-specific IgA and IgG, and levels of anti-RBD IgA correlated with SARS-CoV-2 neutralization. Strong correlations between levels of IgA and IgG to SARS-CoV-2 and seasonal coronaviruses were noted. Conclusions: Our data do not support maternal-to- child TRANS transmission TRANS of SARS-CoV-2 via milk; however, risk of transmission TRANS via breast skin MESHD should be further evaluated. Importantly, milk produced by infected mothers is a source of anti-SARS-CoV-2 IgA and IgG and neutralizes SARS-CoV-2 activity. These results support recommendations to continue breastfeeding during mild-to-moderate maternal COVID-19 illness.

    Seroprevalence SERO and seroconversion rates to SARS-CoV-2 in interns, residents, and medical doctors in a University Hospital in Bogota, Colombia

    Authors: Beatriz Elena Ariza; Yulieth Ximena Torres; Diana Salgado; Magda Cepeda; Carlos Gomez; Julio Cesar Castellanos; Fernando Suarez; Adriana Cuellar; Claudia Cecilia Cardozo; Juana Angel; Manuel Antonio Franco; Timothy D Flietstra; Amy J Schuh; Panayampalli S Satheshkumar; Jasmine M Chaitram; S Michele Owen; M G Finn; Jason M Goldstein; Joel M Montgomery; Christina F Spiropoulou

    doi:10.1101/2020.09.15.20195313 Date: 2020-09-18 Source: medRxiv

    Objectives To determine the prevalence SERO of antibodies to SARS-CoV-2 SERO and the incidence of seroconversion in the first month of follow-up among interns, residents, and medical doctors attending patients at a University Hospital, to explore for associations of seroprevalence SERO and seroconversion with risk factors and symptoms compatible with COVID-19, and to explore the concordance of CLA, LFA, and ELFA. Design or methods We conducted a cross-sectional and a prospective study among medical doctors and medical trainees at Hospital Universitario San Ignacio in Bogota (Colombia) during June, July, and August to assess seroprevalence SERO and seroconversion rates in this population was performed using CLA IgG for SARS-CoV-2. LFA IgG and IgM and ELFA IgM were also determined to explore concordance with CLA IgG. Results At baseline, 8 (2.28% 95%CI 1.16-4.43%) individuals were IgG positive for SARS-CoV-2 by CLA. At the end of the study, 21 (5.98% 95%CI 3.94-8.97%) individuals seroconverted by CLA IgG. In all, 29 individuals had IgG by CLA and of these 11 (3.13% 95%CI 1.76-5.52%) were asymptomatic TRANS. No associations with risk factors for infection MESHD were identified. CLA had moderate concordance with LFA IgG and ELFA, but minimal with LFA IgM. Conclusions Our report is one of the first in Latina America on seroprevalence SERO and seroconversion rates in medical healthcare workers. It emphasizes the importance of avoiding focusing only on symptomatic individuals to screen this population for SARS-CoV-2 infection MESHD, since of all individuals that have evidence of previous infection MESHD many (37.93%) may be pre-symptomatic or asymptomatic TRANS and may contribute to infection MESHD/ disease spread TRANS.

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MeSH Disease
Human Phenotype

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