Corpus overview


Overview

MeSH Disease

Human Phenotype

Allergy (1)


Transmission

Seroprevalence
    displaying 1 - 1 records in total 1
    records per page




    Detection of antibodies to the SARS-CoV-2 SERO spike glycoprotein in both serum SERO and saliva enhances detection of infection

    Authors: Sian E Faustini; Sian E. Jossi; Marisol Perez-Toledo; Adrian Shields; Joel D. Allen; Yasunori Watanabe; Maddy L. Newby; Alex Cook; Carrie R. Willcox; Mahboob Salim; Margaret Goodall; Jennifer L. Heaney; Edith Marcial-Juarez; Gabriella L. Morley; Barbara Torlinska; David C. Wraith; Tonny Veenith; Stephen Harding; Stephen Jolles; Ponsford J Mark; Tim Plant; Aarnoud Huissoon; Matthew K. O'Shea; Benjamin E. Willcox; Mark T. Drayson; Max Crispin; Adam F. Cunningham; Alex G. Richter

    doi:10.1101/2020.06.16.20133025 Date: 2020-06-18 Source: medRxiv

    Background: Detecting antibody SERO responses during and after SARS-CoV-2 infection MESHD is essential in determining the seroepidemiology of the virus and the potential role of antibody SERO in disease. Scalable, sensitive and specific serological assays SERO are essential to this process. The detection of antibody SERO in hospitalized patients with severe disease has proven straightforward; detecting responses in subjects with mild disease and asymptomatic TRANS asymptomatic MESHD infections has proven less reliable. We hypothesized that the suboptimal sensitivity SERO of antibody SERO assays and the compartmentalization of the antibody SERO response may contribute to this effect. Methods: We systemically developed an ELISA assay SERO, optimising different antigens and amplification steps, in serum SERO and saliva from symptomatic and asymptomatic TRANS SARS-CoV-2-infected MESHD subjects. Results: Using trimeric spike glycoprotein, rather than nucleocapsid enabled detection of responses in individuals with low antibody SERO responses. IgG1 and IgG3 predominate to both antigens, but more anti-spike IgG1 than IgG3 was detectable. All antigens were effective for detecting responses in hospitalized patients. Anti-spike, but not nucleocapsid, IgG, IgA and IgM antibody SERO responses were readily detectable in saliva from non-hospitalized symptomatic and asymptomatic TRANS individuals. Antibody SERO responses in saliva and serum SERO were largely independent of each other and symptom reporting. Conclusions. Detecting antibody SERO responses in both saliva and serum SERO is optimal for determining virus exposure and understanding immune responses after SARS-CoV-2 infection MESHD. Funding. This work was funded by the University of Birmingham, the National Institute for Health Research (UK), the NIH National Institute for Allergy HP Allergy MESHD and Infectious Diseases, the Bill and Melinda Gates Foundation and the University of Southampton.

The ZB MED preprint Viewer preVIEW includes all COVID-19 related preprints from medRxiv and bioRxiv, from ChemRxiv, from ResearchSquare, from arXiv and from Preprints.org and is updated on a daily basis (7am CET/CEST).
The web page can also be accessed via API.

Sources


Annotations

All
None
MeSH Disease
Human Phenotype
Transmission
Seroprevalence


Export subcorpus as...

This service is developed in the project nfdi4health task force covid-19 which is a part of nfdi4health.

nfdi4health is one of the funded consortia of the National Research Data Infrastructure programme of the DFG.