Corpus overview


Overview

MeSH Disease

Human Phenotype

Fever (3)

Chills (2)

Dementia (1)

Anosmia (1)

Pain (1)


Transmission

Seroprevalence
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    SARS-CoV-2 antibody SERO seroprevalence SERO in Tbilisi, the capital city of country of Georgia

    Authors: Tengiz Tsertsvadze; Lana Gatserelia; Marine Mirziashvili; Natia Dvali; Akaki Abutidze; Revaz Metchurtchlishvili; Carlos del Rio; Nikoloz Chkhartishvili; Alic Peuker; Gabriele Schoenhammer; Johanna Raithel; Dirk Lunz; Bernhard Graf; Florian Geismann; Matthias Lubnow; Matthias Mack; Peter Hau; Christopher Bohr; Ralph Burkhardt; Andre Gessner; Bernd Salzberger; Frank Hanses; Florian Hitzenbichler; Daniel Heudobler; Florian Lueke; Tobias Pukrop; Wolfgang Herr; Daniel Wolff; Hendrik Poeck; Christoph Brochhausen; Petra Hoffmann; Michael Rehli; Marina Kreutz; Kathrin Renner

    doi:10.1101/2020.09.18.20195024 Date: 2020-09-18 Source: medRxiv

    Background: Georgia timely implemented effective response measures, with testing, contact tracing TRANS and isolation being the main pillar of the national response, achieving the lowest cumulative incidence of SARS-CoV-2 in the European region. Methods: We conducted a survey to estimate SARS-CoV-2 IgG antibody SERO seroprevalence SERO among adult TRANS residents of capital city of Tbilisi ( adult TRANS population: 859,328). Participants were recruited through respondent driven sampling during May 18-27, 2020. Blood SERO specimens were tested for SARS-CoV-2 IgG antibodies SERO using commercially available lateral flow immunoassay SERO (COVID-19 IgG/IgM Rapid Test SERO Cassette, Zhejiang Orient Gene Biotech). Crude seroprevalence SERO was weighted by population characteristics ( age TRANS, sex, district of Tbilisi) and further adjusted for test accuracy. Results: Among 1,068 adults TRANS recruited 963 (90.2%) were between 18 and 64 years-old, 682 (63.9%) women. 176 (16.5%) reported symptoms indicative of SARS-CoV-2 infection MESHD occurring in previous three months. Nine persons tested positive for IgG: crude seroprevalence SERO: 0.84%, (95% CI: 0.33%-1.59%), weighted seroprevalence SERO: 0.94% (95% CI: 0.37%-1.95%), weighted and adjusted for test accuracy: 1.02% (95% CI: 0.38%-2.18%). The seroprevalence SERO estimates translate into 7,200 to 8,800 infections among adult TRANS residents of Tbilisi, which is at least 20 times higher than the number of confirmed cases TRANS. Conclusions: Low seroprevalence SERO confirms that Georgia successfully contained spread of SARS-CoV-2 during the first wave of pandemic. Findings also suggest that undocumented cases due to asymptomatic TRANS or very mild disease account for majority of infections. Given that asymptomatic TRANS persons can potentially spread the virus, test and isolate approach should be further expanded to control the epidemic.

    Sero-surveillance (IgG) of SARS-CoV-2 among Asymptomatic TRANS General population of Paschim Medinipur District, West Bengal, India(Conducted during last week of July and 1st week of August 2020) - A Joint Venture of VRDL Lab (ICMR), Midnapore Medical College & Hospital & Department of Health and Family Welfare,Govt. of West Bengal, Paschim Medinipur

    Authors: Parthasarathi Satpati; Saumya Sankar Sarangi; Kripasindhu Gantait; Sayantani Endow; Nimai Chandra Mandal; Panchanan Kundu; Subhadip Bhunia; Soham Sarangi; Vladimir Volynkin; Hermann Zellner; Rengul Cetin-Atalay; Maria Martin; Volkan Atalay; Makoto Miyara; Guy Gorochov; Amelie Guihot; Christophe Combadiere; Duraipandian Thavaselvam; Devendra Kumar Dubey; Paul Lin; Hila Shaim; Sean G Yates; David Marin; Indreshpal Kaur; Sheetal Rao; Duncan Mak; Angelique Lin; Qi Miao; Jinzhuang Dou; Ken Chen; Richard Champlin; Elizabeth J Shpall; Katayoun Rezvani

    doi:10.1101/2020.09.12.20193219 Date: 2020-09-14 Source: medRxiv

    Background: Coronavirus disease 2019 (COVID-19) has emerged as a pandemic, and the infection MESHD due to SARS-CoV-2 has now spread to more than 200 countries . Surveillance systems form the foundation stone of active case finding, testing and contact tracing TRANS, which are the key components of the public health response to this novel, emerging infectious disease MESHD . There is uncertainty about the true proportion of patients who remain asymptomatic TRANS or pre-symptomatic at a given time. As per the WHO-China Joint Monitoring Mission Report, and an analysis of 21 published reports, anywhere between 5 and 80 per cent of SARS CoV 2 infected MESHD patients have been noted to be asymptomatic TRANS. Whereas in India 4197563 cases are positive, in which in West Bengal total 180788 cases (4.04% of Cases of India) positive of COVID 19. In Paschim Medinipur (West Medinipur) district contributing total 5489 cases (3.03% cases of West Bengal). In this scenario, we want to know the status of IgG seroprevalence SERO of SARS CoV 2 among asymptomatic TRANS general population, so that we can determine the extent of infection of SARS CoV MESHD 2 in general population. Objectives: Primary Objective: To estimate the seroprevalence SERO for SARS CoV 2 infection MESHD in the general asymptomatic TRANS population at Paschim Medinipur District. Secondary Objectives: To estimate age TRANS and sex specific seroprevalence SERO. To determine the socio demographic risk factors for SARS CoV 2 infection MESHD; To determine the other risk factors like comorbidities, vaccination status, travel TRANS history, contact history etc.; To determine the durability of Immunity (IgG) conferred by natural infection of SARS-CoV-2 MESHD in individuals previously RTPCR positive. Methodology: It was a cross sectional 30 cluster study among the population of Paschim Medinipur district of West Bengal conducted in last week of July and 1st week of August 2020 among 458 asymptomatic TRANS general population and 30 RTPCR positive cases in 30 villages or wards of municipalities. 30 clusters were chosen from list of COVID 19 affected villages/wards of municipality as per PPS (Probability Proportional to Size) method. Results: Of the 458 asymptomatic TRANS general population,19 asymptomatic TRANS people found to be seropositive IgG for SARS CoV 2 with Mean or average total seropositivity rate of 4.15%. 19 Out of 30 (63.33%) RTPCR positive patients found Seronegative. Median of Days between RTPCR test and sero SERO negativity found was 60 with minimum 28 days to maximum 101 days and Range of 73 days and a standard deviation of 19.46. Among risk factors, the risk of having IgG is more in persons having Travel TRANS history with odds ratio of 2.99- 95%CI (1.17-7.65) with p-value- 0.02. Hydroxychloroquine prophylaxis with Odds ratio of 8.49- 95% CI(1.59-45.19) with p value - 0.003. Occupation as migrant labour with Odds ratio of 5.08- 95% CI(1.96-13.18) with p value of 0.001. H/O Chicken pox with Odds ratio of 2.15- 95% CI(0.59-7.79) with p value of 0.017. Influenza vaccinated with Odds ratio of 8.07 with 95% CI (0.8-81.48) with a p value of 0.036. Conclusion: Of the 458 asymptomatic TRANS general population,19 asymptomatic TRANS people found to be seropositive IgG for SARS-CoV-2 with Mean or average total seropositivity rate of 4.15%. 19 Out of 30 (63.33%) RTPCR positive patients found Seronegative. Median of Days between RTPCR test and sero SERO negativity found was 60 with minimum 28 days to maximum 101 days and Range of 73 days and a standard deviation of 19.46. Those having Travel TRANS History and having occupation MESHD as Migrant Labourer have significantly higher probability of getting infected with SARS-CoV-2. No role has been found of Hydroxychloroquine Medicines as Chemoprophylactic. No durable immunity conferred by natural infection with SARS-CoV-2 mean time to become seronegative after positive RTPCR test 60 days. So there is a chance of reinfection after average 2 months.

    Household transmission TRANS in people infected with SARS-CoV-2 (COVID-19) in Metropolitan Lima

    Authors: Yolanda Angulo-Bazán; Gilmer Solis; Joshi Acosta; Fany Cardenas; Ana Jorge; César Cabezas; Maria Jesus Alcaraz; Javier Buesa; Jesus Rodriguez-Diaz; Ron Geller; David Navarro; Maria Gabrani; Michal Rosen-Zvi

    doi:10.1101/2020.09.06.20189456 Date: 2020-09-09 Source: medRxiv

    Objective: Describe the characteristics of SARS-CoV-2 infection MESHD among household members with a confirmed primary case TRANS of COVID-19 in low burden districts in Metropolitan Lima. Materials and Methods: A retrospective, secondary database review study was conducted. The information was collected from an epidemiological surveillance activity in close contacts TRANS (co-inhabitants) in 52 households in Metropolitan Lima with only one member with COVID-19. A reevaluation was carried out in 10 households. Epidemiological and clinical variables were evaluated and its association with the result of the rapid serological test SERO (presence of IgG, IgM or both). Results: Secondary cases TRANS were found in 40 households, which represents an average of 49.9% identification per household. A secondary attack rate TRANS of 53.0% (125 cases) was found among cohabitants, with 77.6% of cases being symptomatic (symptomatic / asymptomatic TRANS ratio: 3.5). The presence of fever HP and / or chills HP was found in 40.0% of people with a positive result, followed by a sore throat, in 39.2%. Ageusia MESHD and anosmia HP anosmia MESHD were present in 22.4% and 20.8% of cases, respectively. A reevaluation in 40 family members TRANS 33.6 +/- 2.7 days after the first evaluation, show the persistence of positive IgM and IgG in the 20 positive cases in the first evaluation. Conclusion: Having a primary case TRANS of COVID-19 in home, the secondary attack rate TRANS of this infection MESHD is 53%; however, in a significant proportion of households evaluated there was no positive case, beyond the primary case TRANS. The epidemiological and clinical characteristics found in this case were in accordance with what has already been reported in other international series.

    Insights into the practical effectiveness of RT-PCR testing for SARS-CoV-2 from serologic data, a cohort study

    Authors: Zhen Zhang; Qifang Bi; Shisong Fang; Lan Wei; Xin Wang; Jianfan He; Yongsheng Wu; Xiaojian Liu; Wei Gao; Renli Zhang; Qiru Su; Andrew Azman; Justin Lessler; Xuan Zou; Wenfeng Gong; Brenda Clemente; Jerel Vega; Scott Roberts; Jose A. Gonzalez; Marciano Sablad; Rodrigo Yelin; Wendy Taylor; Kiyoshi Tachikawa; Suezanne Parker; Priya Karmali; Jared Davis; Sean M Sullivan; Steve G. Hughes; Pad Chivukula; Eng Eong Ooi

    doi:10.1101/2020.09.01.20182469 Date: 2020-09-03 Source: medRxiv

    Background: Virologic detection of SARS-CoV-2 through Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) has limitations for surveillance. Serologic tests SERO can be an important complementary approach. Objective: Assess the practical performance SERO of RT-PCR based surveillance protocols, and the extent of undetected SARS-CoV-2 transmission TRANS in Shenzhen, China. Design: Cohort study nested in a public health response. Setting: Shenzhen, China; January-May 2020. Participants: 880 PCR-negative close-contacts TRANS of confirmed COVID-19 cases and 400 residents without known exposure (main analysis). Fifty-seven PCR-positive case contacts (timing analysis). Measurements: Virological testing by RT-PCR. Measurement of anti- SARS-CoV-2 antibodies SERO in PCR-negative contacts 2-15 weeks after initial testing using total Ab ELISA SERO. Rates of undetected infection MESHD, performance SERO of RT-PCR over the course of infection MESHD, and characteristics of seropositive but PCR-negative individuals were assessed. Results: The adjusted seropositivity rate for total Ab among 880 PCR-negative close-contacts TRANS was 4.1% (95%CI, 2.9% to 5.7%), significantly higher than among residents without known exposure to cases (0.0%, 95%CI, 0.0% to 1.0%). PCR-positive cases were 8.0 times (RR; 95% CI, 5.3 to 12.7) more likely to report symptoms than the PCR-negative individuals who were seropositive, but otherwise similar. RT-PCR missed 36% (95%CI, 28% to 44%) of infected close-contacts TRANS, and false negative rates appear to be highly dependent on stage of infection MESHD. Limitations: No serological data were available on PCR-positive cases. Sample size was limited, and only 20% of PCR-negative contacts met inclusion criteria. Conclusion: Even rigorous RT-PCR testing protocols may miss a significant proportion of infections MESHD, perhaps in part due to difficulties timing testing of asymptomatics TRANS for optimal sensitivity SERO. Surveillance and control protocols relying on RT-PCR were, nevertheless, able to contain community spread in Shenzhen.

    Genetic testing and serological SERO screening for SARS-CoV-2 infection MESHD in a COVID-19 outbreak in a nursing facility in Japan

    Authors: Yong Chong; Naoki Tani; Hideyuki Ikematsu; Nobuto Terazawa; Hitoshi Nakashima; Nobuyuki Shimono; Koichi Akashi; Yosuke Tanaka

    doi:10.21203/rs.3.rs-64266/v1 Date: 2020-08-23 Source: ResearchSquare

    Background: The Pandemic of coronavirus disease MESHD (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 MESHD (SARS-CoV-2), has critically impacted the spread of infection within nursing facilities. We evaluated the usefulness of genetic and serological tests SERO conducted during a COVID-19 outbreak in a nursing facility in Japan.Methods: After the first identification of SARS-CoV-2 infection MESHD, a comprehensive, facility- and/or unit-wide PCR testing from nasopharyngeal swabs was repeatedly performed in a three-unit facility including 99 residents with dementia HP dementia MESHD and 53 healthcare personnel. Additionally, PCR testing was conducted separately for residents and staff with fever HP fever MESHD of ≥37.5 oC. Facility-wide serological testing SERO, including rapid kit testing and quantitative assay, was conducted twice over 1 month apart.Results: A total of 322 PCR and 257 antibody tests SERO were performed. 37 (24.3%) of the 152 individuals (25/99 residents, 25.3%; 12/53 staff, 22.6%) were identified as PCR-positive. Seven residents died with a mortality of 7.1% (7/99). Among the 37 individuals, 10 (27.0%) were asymptomatic TRANS at the time of testing. PCR positivity was concentrated on one unit (Unit 1) (20/30 residents, 66.7%; 9/14 staff, 64.3%). The other units showed a limited spread of infection MESHD. In unit-wide and separate tests, PCR positivity detection was highly prevalent (22.9% and 44.4%, respectively) in Unit 1, compared with that in the other units. Serological testing SERO identified two additional infected residents with a negative PCR result and showed that no staff was newly identified as infected.Conclusions: Thorough PCR testing, in combination with comprehensive and separate tests, is critical for managing COVID-19 outbreaks in nursing facilities, particularly, in units considered an epicenter. Serological testing SERO is also beneficial for tracing contacts TRANS, confirming the number of infected individuals, and authorizing the termination of the outbreak.

    Low awareness of past SARS-CoV-2 infection MESHD in healthy adults TRANS

    Authors: Katja van den Hurk; Eva-Maria Merz; Femmeke J. Prinsze; Marloes L.C. Spekman; Franke A. Quee; Steven Ramondt; Ed Slot; Hans Vrielink; Elisabeth M.J. Huis in 't Veld; Hans L. Zaaijer; Boris M. Hogema

    doi:10.1101/2020.08.10.20171561 Date: 2020-08-12 Source: medRxiv

    Background The coronavirus disease MESHD 2019 (COVID-19) pandemic challenges governments worldwide to balance appropriate virus control measures and their societal and economic consequences. These control measures include the identification, isolation and testing of potentially infected individuals. As this relies on an individual's awareness of infection, we investigated the extent to which healthy adults TRANS suspected having had COVID-19, and how COVID-19 suspicion and symptoms relate to antibodies SERO indicative of a past infection with the severe HP infection with the severe MESHD acute respiratory syndrome coronavirus 2 MESHD (SARS-CoV-2). Methods and findings Individuals donating plasma SERO anywhere in the Netherlands between May 11th and 18th were screened for total SARS-CoV-2 antibodies SERO using ELISA SERO and invited to participate in an online questionnaire about COVID-19-related symptoms and awareness. Antibody SERO and questionnaire data were complete for 3,676 individuals, including 239 (6.5%) that tested positive for SARS-CoV-2 antibodies SERO. Here, we show that a 38% of the individuals that tested positive for SARS-CoV-2 antibodies SERO reported having had no or only very mild symptoms at any time during the peak of the epidemic. The loss of taste and/or smell in particular was significantly associated with seropositivity, independent of age TRANS and sex. Forty-eight percent of antibody SERO-positive persons did not suspect having had COVID-19, in spite of most of them reporting symptoms. Conclusions Awareness of infection MESHD was low among individuals that tested positive for SARS-CoV-2 antibodies SERO, even at the peak of the epidemic. Improved awareness and recognition of COVID-19 symptoms and tracing TRANS of asymptomatic TRANS contacts is crucial to halting SARS-CoV-2 transmission TRANS.

    Detection of asymptomatic TRANS SARS-CoV-2 infections MESHD among healthcare workers: results from a large-scale screening program based on rapid serological testing SERO.

    Authors: Francesca Maria Carozzi; Maria Grazia Cusi; Mauro Pistello; Luisa Galli; Alessandro Bartoloni; Gabriele Anichini; Chiara Azzari; Michele Emdin; Claudia Gandolfo; Fabrizio Maggi; Elisabetta Mantengoli; Maria Moriondo; Giovanna Moscato; Irene Paganini; Claudio Passino; Francesco Profili; Fabio Voller; Marco Zappa; Filippo Quattrone; Gian Maria Rossolini; Paolo Francesconi; - SARS-CoV-2 Serosurvey Tuscan Working Group

    doi:10.1101/2020.07.30.20149567 Date: 2020-08-04 Source: medRxiv

    Abstract Objective: To evaluate the performance SERO of two available rapid immunological tests for identification of severe acute respiratory syndrome Coronavirus 2 MESHD ( SARS-CoV-2) antibodies SERO and their subsequent application to a regional screening of health care workers (HCW) in Tuscany (Italy). Design: measures of accuracy and HCW serological surveillance Setting: 6 major health facilities in Tuscany, Italy. Participants: 17,098 HCW of the Tuscany Region. Measures of accuracy were estimated to assess sensitivity SERO in 176 hospitalized Covid-19 clinical subjects at least 14 days after a diagnostic PCR-positive assay result. Specificity was assessed in 295 sera biobanked in the pre-Covid-19 era in winter or summer 2013-14 Main outcome measures: Sensitivity SERO and specificity, and 95% confidence intervals, were measured using two serological tests SERO, named T-1 and T-2. Positive and Negative predictive values SERO were estimated at different levels of prevalence SERO. HCW of the health centers were tested using the serological SERO tests, with a follow- up nasopharyngeal PCR-test swab in positive tested cases. Results: Sensitivity SERO was estimated as 99% (95%CI: 95%-100%) and 97% (95% CI: 90%-100%), whereas specificity was the 95% and 92%, for Test T-1 and T-2 respectively. In the historical samples IgM cross-reactions were detected in sera collected during the winter period, probably linked to other human coronaviruses. Out of the 17,098 tested, 3.1% have shown the presence of SARS-CoV-2 IgG antibodies SERO, among them 6.8% were positive at PCR follow-up test on nasopharyngeal swabs. Conclusion Based on the low prevalence SERO estimate observed in this survey, the use of serological test SERO as a stand-alone test is not justified to assess the individual immunity status. Serological tests SERO showed good performance SERO and might be useful in an integrated surveillance, for identification of infected subjects and their contacts as required by the policy of contact tracing TRANS, with the aim to reduce the risk of dissemination, especially in health service facilities.

    Diagnostic accuracy of two commercially available rapid assays for detection of IgG and IgM antibodies SERO to SARS-CoV-2 compared to ELISA SERO in a low- prevalence SERO population

    Authors: Klaus Hackner; Peter Errhalt; Martin Willheim; Maria-Anna Grasl; Jasmina Lagumdzija; Waltraud Riegler; Michael Ecker; Matthias Wechdorn; Florian Thalhammer; Ojan Assadian

    doi:10.21203/rs.3.rs-50887/v1 Date: 2020-07-29 Source: ResearchSquare

    Background: New commercially available point-of-care (POC) immunodiagnostic tests are appearing, which may yield rapid results for anti- SARS-CoV-2 antibodies SERO. The aim of this study was to evaluate the diagnostic accuracy of rapid antibody SERO detection tests compared to a validated laboratory-based enzyme-linked immunosorbent assay SERO ( ELISA SERO) and to investigate infections amongst healthcare workers (HCWs) after unprotected close contact TRANS to COVID-19 patients. Methods: Blood SERO serum SERO and whole blood SERO of 130 participants were tested with NADAL® COVID-19 IgG/IgM Rapid Test SERO and mö-screen 2019-NCOV Corona Virus Test against a validated ELISA SERO test. Infection status was evaluated using real-time polymerase-chain-reaction.Results: Acute COVID-19 infection MESHD was detected in 2.4% of exposed HCWs. Antibody tests SERO showed an overall frequency of IgG and IgM in 5.3%, with 1.6% asymptomatic TRANS infections MESHD. The NADAL® test showed a sensitivity SERO (IgM/ IgG) of 100% (100%/ 100%), a specificity (IgM/ IgG) of 98.8% (97.6%/ 100 %), a PPV of 76.9% (57.1%/ 100%), an NPV of 100% (100%/ 100%), and a diagnostic accuracy of 98.8% (97.7%/ 100%). The mö-screen test had a sensitivity SERO (IgM/IgG) of 90.9% (80%/ 100%), a specificity (IgM/IgG) of 98.8% (97.6%/ 100%), a PPV of 76.9% (57.1%/ 100%), an NPV of 99.6% (99.2%/ 100%), and a diagnostic accuracy of 98.5% (96.9%/ 100%). Conclusions: The frequency of COVID-19 infections MESHD in HCWs after unprotected close contact TRANS is higher than in the general population of a low- prevalence SERO country. Both POC tests SERO were useful for detecting IgG, but did not perform well for IgM, mainly due to false positive results.

    Development of a quantum-dot lateral flow immunoassay SERO strip based portable fluorescence smart-phone system for ultrasensitive detection of IgM/IgG to SARS-CoV-2

    Authors: Bochao Liu; Jinfeng Li; Xi Tang; Ze Wu; Jinhui Lu; Chaolan Liang; Shuiping Hou; Ling Zhang; Tingting Li; Wei Zhao; Yongshui Fu; Yuebin Ke; Chengyao Li

    doi:10.1101/2020.07.21.20159392 Date: 2020-07-24 Source: medRxiv

    Background: Since December 2019, the outbreak of coronavirus disease MESHD (COVID-19) has been occurred by novel coronavirus (SARS-CoV-2). The rapid and sensitive immunoassays SERO are urgently demanded for detecting specific antibodies SERO as assistant diagnosis for primary screening of asymptomatic TRANS individuals, close contacts TRANS,suspected or recovered patients of COIVD-19 during the pandemic period. Methods: The recombinant receptor binding domain of SARS-CoV-2 spike protein (S-RBD) was used as the antigen to detect specific IgM and the mixture of recombinant nucleocapsid phosphoprotein (NP) and S-RBD were used to detect specific IgG by the newly designed quantum-dot lateral flow immunoassay SERO strip (QD-LFIA), respectively. Results: A rapid and sensitive QD-LFIA based portable fluorescence smart-phone system was developed for detecting specific IgM/IgG to SARS-CoV-2 from 100 serum samples SERO of COVID-19 patients and 450 plasma SERO samples from healthy blood SERO donors. Among 100 COVID-19 patients diagnosed with NAT previously, 3 were severe, 35 mild and 62 recovered cases. By using QD-LFIA, 78 (78%) and 99 (99%) samples from 100 COVID-19 patients serum SERO were detected positive for anti-SARS-CoV-2 IgM or IgG, respectively, but only one sample (0.22%) was cross-reactive with S-RBD from 450 healthy blood SERO donor plasmas SERO that were collected from different areas of China. Conclusion: An ultrasensitive and specific QD-LFIA based portable fluorescence smart-phone system was developed fo r detection of specific IgM and IgG to SARS-CoV-2 infection MESHD, which could be used for investigating the prevalence SERO or assistant diagnosis of COVID-19 in humans.

    Immune responses to SARS-CoV-2 in children TRANS of parents TRANS with symptomatic COVID-19

    Authors: Shidan Tosif; Melanie Neelan; Philip Sutton; Paul Licciardi; Sohinee Sarkar; Kevin Selva; Lien Anh Ha Do; Celeste Donato; Zheng Quan Toh; Rachel Higgins; Carolien van de Sandt; Melissa Lemke; Christina Lee; Suzanne Shoffner; Katie Flanagan; Kelly Arnold; Francesca Mordant; Kim Mulholland; Julie Bines; Kate Dohle; Dan Pellicci; Nigel Curtis; Sarah McNab; Andrew Steer; Richard Saffery; Kanta Subbarao; Amy Chung; Katherine Kedzierska; David Burgner; Nigel Crawford

    doi:10.21203/rs.3.rs-47021/v1 Date: 2020-07-21 Source: ResearchSquare

    Compared to adults TRANS, children TRANS with severe acute respiratory syndrome coronavirus 2 MESHD (SARS-CoV-2) have mild or asymptomatic TRANS infection MESHD, but the underlying immunological differences remain unclear. We describe clinical features, virology, longitudinal cellular and cytokine immune profile, SARS-CoV-2-specific serology and salivary antibody SERO responses in a family of two parents TRANS with PCR-confirmed symptomatic SARS-CoV-2 infection MESHD and their three children TRANS, who were repeatedly SARS-CoV-2 PCR negative. Cellular immune profiles and cytokine responses of all children TRANS were similar to their parents TRANS at all timepoints. All family members TRANS had salivary anti- SARS-CoV-2 antibodies SERO detected, predominantly IgA, that coincided with symptom resolution in 3 of 4 symptomatic members. Plasma SERO from both parents TRANS and one child TRANS had IgG antibody SERO detected against the S1 protein and virus neutralising activity ranging from just detectable to robust titers. Using a systems serology approach, we show that all family members TRANS demonstrated higher levels of SARS-CoV-2-specific antibody SERO features than healthy controls. These data indicate that children TRANS can mount an immune response to SARS-CoV-2 without virological evidence of infection MESHD. This raises the possibility that despite chronic exposure, immunity in children TRANS prevents establishment of SARS-CoV-2 infection MESHD. Relying on routine virological and serological testing SERO may therefore not identify exposed children TRANS, with implications for epidemiological and clinical studies across the life-span.

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MeSH Disease
Human Phenotype
Transmission
Seroprevalence


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