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HGNC Genes

SARS-CoV-2 proteins

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    The N-terminal domain of spike glycoprotein PROTEIN mediates SARS-CoV-2 infection MESHD by associating with L-SIGN HGNC and DC-SIGN

    Authors: Wai Tuck Soh; Yafei Liu; Emi E Nakayama; Chikako Ono; Shiho Torii; Hironori Nakagami; Yoshiharu Matsuura; Tatsuo Shioda; Hisashi Arase; Reid Simon; Ivan Grishagin; Laura Brovold; Ewy A Mathé; Matthew D Hall; Samuel G Michael; Alexander G Godfrey; Jordi Mestres; Lars J Jensen; Tudor I Oprea; Isabel Crooker; Sara Y Del Valle; Guido Espana; Geoffrey Fairchild; Richard C Gerkin; Timothy C Germann; Quanquan Gu; Xiangyang Guan; Lihong Guo; Gregory R Hart; Thomas J Hladish; Nathaniel Hupert; Daniel Janies; Cliff C Kerr; Daniel J Klein; Eili Klein; Gary Lin; Carrie Manore; Lauren Ancel Meyers; John Mittler; Kunpeng Mu; Rafael C NUNez; Rachel Oidtman; Remy Pasco; Ana Pastore y Piontti Pastore y Piontti; Rajib Paul; Carl AB Pearson; Dianela Perdomo; T Alex Perkins; Kelly Pierce; Alexander N Pillai; Rosalyn Cherie Rael; Katherine Rosenfeld; Chrysm Watson Ross; Julie A Spencer; Arlin B Stoltzfus; Kok Ben Toh; Shashaank Vattikuti; Alessandro Vespignani; Lingxiao Wang; Lisa White; Pan Xu; Yupeng Yang; Osman N Yogurtcu; Weitong Zhang; Yanting Zhao; Difan Zou; Matthew Ferrari; David Pannell; Michael Tildesley; Jack Seifarth; Elyse Johnson; Matthew Biggerstaff; Michael Johansson; Rachel B Slayton; John Levander; Jeff Stazer; Jessica Salermo; Michael C Runge

    doi:10.1101/2020.11.05.369264 Date: 2020-11-05 Source: bioRxiv

    The widespread occurrence of SARS-CoV-2 has had a profound effect on society and a vaccine is currently being developed. Angiotensin-converting enzyme 2 HGNC ( ACE2 HGNC) is the primary host cell receptor that interacts with the receptor-binding domain (RBD) of the SARS-CoV-2 spike PROTEIN protein. Although pneumonia MESHD is the main symptom in severe cases of SARS-CoV-2 infection MESHD, the expression levels of ACE2 HGNC in the lung is low, suggesting the presence of another receptor for the spike protein PROTEIN. In order to identify the additional receptors for the spike protein PROTEIN, we screened a receptor for the SARS-CoV-2 spike PROTEIN protein from the lung cDNA library. We cloned L-SIGN HGNC as a specific receptor for the N-terminal domain ( NTD HGNC) of the SARS-CoV-2 spike PROTEIN protein. The RBD of the spike protein PROTEIN did not bind to L-SIGN HGNC. In addition, not only L-SIGN HGNC but also DC-SIGN, a closely related C-type lectin receptor HGNC to L-SIGN HGNC, bound to the NTD HGNC of the SARS-CoV-2 spike PROTEIN protein. Importantly, cells expressing L-SIGN HGNC and DC-SIGN were both infected by SARS-CoV-2. Furthermore, L-SIGN HGNC and DC-SIGN induced membrane fusion by associating with the SARS-CoV-2 spike PROTEIN protein. Serum antibodies from infected patients and a patient-derived monoclonal antibody against NTD HGNC inhibited SARS-CoV-2 infection of L-SIGN MESHD L-SIGN HGNC or DC-SIGN expressing cells. Our results highlight the important role of NTD HGNC in SARS-CoV-2 dissemination through L-SIGN HGNC and DC-SIGN and the significance of having anti- NTD HGNC neutralizing antibodies in antibody-based therapeutics.

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MeSH Disease
HGNC Genes
SARS-CoV-2 Proteins


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