Corpus overview


MeSH Disease

HGNC Genes

SARS-CoV-2 proteins

ProteinS (2)

ORF3a (1)

ORF8 (1)

ProteinE (1)

ProteinN (1)


SARS-CoV-2 Proteins
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    Long-read sequencing of SARS-CoV-2 reveals novel transcripts and a diverse complex transcriptome landscape.

    Authors: Jennifer Li-Pook-Than; Selene Banuelos; Alexander Honkala; Malaya K Sahoo; Benjamin A Pinsky; Michael P Snyder

    doi:10.1101/2021.03.05.434150 Date: 2021-03-06 Source: bioRxiv

    Severe Acute Respiratory Syndrome Coronavirus 2 MESHD, SARS-CoV-2 ( COVID-19 MESHD), is a positive single-stranded RNA virus with a 30 kb genome that is responsible for the current pandemic. To date, the genomes of global COVID-19 MESHD variants have been primarily characterized via short-read sequencing methods. Here, we devised a long-read RNA (IsoSeq) sequencing approach to characterize the COVID-19 MESHD transcript landscape and expression of its [~]27 coding regions. Our analysis identified novel COVID-19 MESHD transcripts including a) a short [~]65-70 nt 5-UTR fused to various downstream ORFs encoding accessory proteins such as the envelope PROTEIN, ORF 8, and ORF 9 HGNC ( nucleocapsid) proteins PROTEIN, that are relatively highly expressed, b) novel SNVs that are differentially expressed, whereby a subset are suggestive of partial RNA editing events, and c) SNVs at functional sites, whereby at least one is associated with a differentially expressed spike protein PROTEIN isoform. These previously uncharacterized COVID-19 MESHD isoforms, expressed genes, and gene variants were corroborated using ddPCR. Understanding this transcriptional complexity may help provide insight into the biology and pathogenicity of SARS-CoV-2 compared to other coronaviruses.

    A SARS-CoV-2 lineage A variant (A.23.1) with altered spike has emerged and is dominating the current Uganda epidemic MESHD

    Authors: Daniel Lule Bugembe; My V.T. Phan; Isaac Ssewanyana; Patrick Semanda; Hellen Nansumba; Beatrice Dhaala; Susan Nabadda; Aine O'Toole; Andrew Rambaut; Pontiano Kaleebu; Matthew Cotten

    doi:10.1101/2021.02.08.21251393 Date: 2021-02-11 Source: medRxiv

    The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) was first detected in March 2020 in Uganda. Recently the epidemic showed a shift of SARS-CoV-2 variant distribution and we report here newly emerging A sub-lineages, A.23 and A.23.1, encoding replacements in the spike protein PROTEIN, nsp6, ORF8 PROTEIN and ORF9 HGNC, with A.23.1 the major virus lineage now observed in Kampala. Although the clinical impact of the A.23.1 variant is not yet clear it is essential to continue careful monitoring of this variant, as well as rapid assessment of the consequences of the spike protein PROTEIN changes for vaccine efficacy.

    A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterization in different cell types

    Authors: Stéphanie Miserey-Lenkei; Katarina Trajkovic; Alenka Čopič; Pallavi Mathur; Kristine Schauer; bruno Goud; Véronique Albanèse; Kristina Dietert; Michael Mülleder; Vadim Farztdinov; Benedikt Obermayer; Sandra-Maria Wienhold; Sandro Andreotti; Thomas Höfler; Birgit Sawitzki; Christian Drosten; Leif Erik Sander; Norbert Suttorp; Markus Ralser; Dieter Beule; Achim Dieter Gruber; Christine Goffinet; Markus Landthaler; Jakob Trimpert; Martin Witzenrath

    doi:10.1101/2020.12.19.423586 Date: 2020-12-19 Source: bioRxiv

    Comprehensive libraries of plasmids for SARS-CoV-2 proteins MESHD with various tags (e.g. Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein-protein interactions between the SARS-CoV-2 virus and host proteins. To facilitate further cellular investigations, notably by imaging techniques, we present here a large library of SARS CoV-2 protein constructs fused with green and red fluorescent proteins and their initial characterization in various human cell lines including lung epithelial cell models (A549, BEAS-2B), as well as in budding yeast. The localization of a few SARS-CoV-2 proteins matches their proposed interactions with host proteins. These include the localization of Nsp13 to the centrosome, Orf3a PROTEIN to late endosomes, and Orf9 HGNCb to mitochondria.

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MeSH Disease
HGNC Genes
SARS-CoV-2 Proteins

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