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HGNC Genes

SARS-CoV-2 proteins

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    Structural basis to design multi-epitope vaccines against Novel Coronavirus 19 ( COVID19 MESHD) infection, the ongoing pandemic emergency: an in silico approach

    Authors: Sukrit Srivastava; Sonia Verma; Mohit Kamthania; Rupinder Kaur; Ruchi Kiran Badyal; Ajay Kumar Saxena; Ho-Joon Shin; Michael Kolbe; Kailash Pandey

    doi:10.1101/2020.04.01.019299 Date: 2020-04-03 Source: bioRxiv

    The 2019 novel coronavirus ( COVID19 MESHD / Wuhan coronavirus), officially named as Severe Acute Respiratory Syndrome Coronavirus 2 MESHD (SARS-CoV-2), is a positive-sense single-stranded RNA coronavirus. SARS-CoV-2 causes the contagious COVID19 MESHD disease also known as 2019-nCoV acute respiratory disease MESHD and has led to the ongoing 2019-20 pandemic COVID19 MESHD outbreak. The effective counter measures against SARS-CoV-2 infection MESHD require the design and development of specific and effective vaccine candidate. In the present study, we have screened and shortlisted 38 CTL, 33 HTL MESHD and 12 B cell epitopes from the eleven Protein sequences of SARS-CoV-2 by utilizing different in silico tools. The screened epitopes were further validated for their binding with their respective HLA allele binders and TAP HGNC ( Transporter associated with antigen processing) HGNC molecule by molecular docking. The shortlisted screened epitopes were further utilized to design novel two multi-epitope vaccines (MEVs) composed of CTL, HTL MESHD and B cell epitopes overlaps with potential to elicit humoral as well as cellular immune response against SARS-CoV-2. To enhance the immune response for our vaccine design, truncated (residues 10-153) Onchocerca volvulus activation-associated secreted protein-1 (Ov-ASP-1) has been utilized as an adjuvant at N terminal of both the MEVs. Further molecular models for both the MEVs were prepared and validated for their stable molecular interactions with Toll-Like Receptor 3 HGNC ( TLR 3 HGNC). The codon-optimized cDNA of both the MEVs were further analyzed for their potential of high level of expression in a human cell line. The present study is very significant in terms of molecular designing of prospective CTL and HTL MESHD vaccine against SARS-CoV-2 infection MESHD with the potential to elicit cellular as well as humoral immune response. (SARS-CoV-2), Coronavirus, Human Transporter associated with antigen processing (TAP) HGNC, Toll-Like Receptor (TLR), Epitope, Immunoinformatics, Molecular Docking, Molecular dynamics simulation, Multi-epitope Vaccine Graphical abstractThe designed CTL (Cytotoxic T lymphocyte) and HTL (Helper T lymphocyte) multi-epitope vaccines (MEV) against COVID19 MESHD infection. Both the CTL and HTL MEV models show a very stable and well fit conformational complex formation tendency with the Toll like receptor 3 HGNC. CTL and HTL MEVs: ribbon; Toll like receptor 3 HGNC: gray cartoon; Adjuvant [truncated (residues 10-153) Onchocerca volvulus activation-associated secreted protein-1]: orange ribbon regions; Epitopes: cyan ribbons regions; 6xHis Tag: magenta ribbon regions. O_FIG O_LINKSMALLFIG WIDTH=87 HEIGHT=200 SRC="FIGDIR/small/019299v2_ufig1.gif" ALT="Figure 1"> View larger version (28K): org.highwire.dtl.DTLVardef@6cb749org.highwire.dtl.DTLVardef@1752d54org.highwire.dtl.DTLVardef@1f2fc16org.highwire.dtl.DTLVardef@18415a9_HPS_FORMAT_FIGEXP M_FIG C_FIG

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MeSH Disease
HGNC Genes
SARS-CoV-2 Proteins


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