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HGNC Genes

SARS-CoV-2 proteins

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    Human Embryonic Stem Cell-derived Lung Organoids: a Model for SARS-CoV-2 Infection MESHD and Drug Test

    Authors: Rongjuan Pei; Jianqi Feng; Yecheng Zhang; Hao Sun; Lian Li; Xuejie Yang; Jiangping He; Shuqi Xiao; Jin Xiong; Ying Lin; Kun Wen; Hongwei Zhou; Jiekai Chen; Zhili Rong; Xinwen Chen

    doi:10.1101/2020.08.10.244350 Date: 2020-08-12 Source: bioRxiv

    The coronavirus disease 2019 MESHD ( COVID-19 MESHD) pandemic is caused by infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is spread primary via respiratory droplets and infects the lungs MESHD. Currently widely used cell lines and animals are unable to accurately mimic human physiological conditions because of the abnormal status of cell lines (transformed or cancer MESHD cells) and species differences between animals and humans. Organoids are stem cell-derived self-organized three-dimensional culture in vitro and model the physiological conditions of natural organs. Here we demonstrated that SARS-CoV-2 infected MESHD and extensively replicated in human embryonic stem cells (hESCs)-derived lung organoids, including airway and alveolar MESHD organoids. Ciliated cells, alveolar type 2 MESHD ( AT2 MESHD AT2 HGNC) cells and rare club cells were virus target cells. Electron microscopy captured typical replication, assembly and release ultrastructures and revealed the presence of viruses within lamellar bodies in AT2 HGNC AT2 MESHD cells. Virus infection MESHD induced more severe cell death in alveolar MESHD organoids than in airway organoids. Additionally, RNA-seq revealed early cell response to SARS-CoV-2 infection MESHD and an unexpected downregulation of ACE2 HGNC mRNA. Further, compared to the transmembrane protease, serine 2 ( TMPRSS2 HGNC) inhibitor camostat, the nucleotide analog prodrug Remdesivir potently inhibited SARS-CoV-2 replication in lung organoids. Therefore, human lung organoids can serve as a pathophysiological model for SARS-CoV-2 infection MESHD and drug discovery.

    Single-cell RNA expression profiling of ACE2 HGNC, the putative receptor of Wuhan 2019-nCov

    Authors: Yu Zhao; Zixian Zhao; Yujia Wang; Yueqing Zhou; Yu Ma; Wei Zuo

    doi:10.1101/2020.01.26.919985 Date: 2020-01-26 Source: bioRxiv

    A novel coronavirus SARS-CoV-2 MESHD was identified in Wuhan, Hubei Province, China in December of 2019. According to WHO report, this new coronavirus has resulted in 76,392 confirmed infections and 2,348 deaths in China by 22 February, 2020, with additional patients being identified in a rapidly growing number internationally. SARS-CoV-2 was reported to share the same receptor, Angiotensin-converting enzyme 2 HGNC ( ACE2 HGNC), with SARS-CoV MESHD. Here based on the public database and the state-of-the-art single-cell RNA-Seq technique, we analyzed the ACE2 HGNC RNA expression profile in the normal human lungs. The result indicates that the ACE2 HGNC virus receptor expression is concentrated in a small population of type II alveolar MESHD cells ( AT2 HGNC). Surprisingly, we found that this population of ACE2 HGNC-expressing AT2 HGNC also highly expressed many other genes that positively regulating viral entry, reproduction and transmission. This study provides a biological background for the epidemic investigation of the COVID-19 MESHD, and could be informative for future anti- ACE2 HGNC therapeutic strategy development.

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MeSH Disease
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