Corpus overview


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MeSH Disease

HGNC Genes

claudin 4 (1)


SARS-CoV-2 proteins

There are no SARS-CoV-2 protein terms in the subcorpus


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    Establishment of a reverse genetics system for SARS-CoV-2 using circular polymerase extension reaction

    Authors: Shiho Torii; Chikako Ono; Rigel Suzuki; Yuhei Morioka; Itsuki Anzai; Yuzy Fauzyah; Yusuke Maeda; Wataru Kamitani; Takasuke Fukuhara; Yoshiharu Matsuura; Bryn Tennant; Darcy Herrity; Ana C Filipe; Daniel G Streicker; Brian J Willett; Pablo R Murcia; Ruth F Jarrett; David L Robertson; William Weir; - COVID-19 Genomics UK Consortium; Raiees Andrabi

    doi:10.1101/2020.09.23.309849 Date: 2020-09-23 Source: bioRxiv

    Severe acute respiratory syndrome coronavirus 2 MESHD (SARS-CoV-2) has been identified as the causative agent of coronavirus disease 2019 MESHD ( COVID-19 MESHD). While the development of specific treatments and a vaccine is urgently needed, functional analyses of SARS-CoV-2 have been limited by the lack of convenient mutagenesis methods. In this study, we established a PCR-based, bacterium-free method to generate SARS-CoV-2 infectious clones. Recombinant SARS-CoV-2 could be rescued at high titer with high accuracy after assembling 10 SARS-CoV-2 cDNA fragments by circular polymerase extension reaction ( CPER HGNC) and transfection of the resulting circular genome into susceptible cells. Notably, the construction of infectious clones for reporter viruses and mutant viruses could be completed in two simple steps: introduction of reporter genes or mutations into the desirable DNA fragments (~5,000 base pairs) by PCR and assembly of the DNA fragments by CPER HGNC. We hope that our reverse genetics system will contribute to the further understanding of SARS-CoV-2.

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MeSH Disease
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SARS-CoV-2 Proteins


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