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SARS-CoV-2 proteins

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    Production of SARS-CoV-2 virus-like particles in insect cells

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    doi:10.1101/2021.01.30.428979 Date: 2021-02-01 Source: bioRxiv

    Coronavirus disease ( COVID-19 MESHD) causes a serious threat to human health. To production of SARS-COV-2 virus-like particles (VLPs) in insect cells for vaccine development and scientific research. The E, M and S genes were cloned into multiple cloning sites of the new triple expression plasmid with one p10 HGNC promoter, two pPH HGNC promoters and three multiple cloning sites. The plasmid was transformed into DH10 BacTM Escherichia coli competent cells to obtain recombinant bacmid. Then the recombinant bacmid was transfected in ExpiSf9 insect cells to generate recombinant baculovirus. After ExpiSf9 infected with the recombinant baculovirus, the E, M, and S protein PROTEIN co-expressed in insect cells. Finally, SARS-CoV-2 VLPs MESHD were self-assembled in insect cells after infection. The morphology and the size of SARS-CoV-2 VLPs MESHD are similar to the native virions.

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MeSH Disease
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