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SARS-CoV-2 proteins

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    Ultrapotent SARS-CoV-2 neutralizing antibodies with protective efficacy against newly emerged mutational variants

    Authors: Tingting Li; XiaoJian Han; Chenjian Gu; Hangtian Guo; Huajun Zhang; Yingming Wang; Chao Hu; Kai Wang; Fangjiang Liu; Feiyang Luo; Yanan Zhang; Jie Hu; Wang wang; Shenglong Li; Yanan Hao; Meiying Shen; Jingjing Huang; Yingyi Long; Shuyi Song; Ruixin Wu; Song Mu; Qian Chen; Fengxia Gao; Jianwei Wang; Shunhua Long; Luo Li; Yang Wu; Yan Gao; Wei Xu; Xia Cai; Di Qu; Zherui Zhang; Hongqing Zhang; Na Li; Qingzhu Gao; guiji Zhang; Changlong He; Wei Wang; Xiaoyun Ji; Ni Tang; Zhenghong Yuan; Youhua Xie; Bo Zhang; Haitao Yang; Ailong Huang; Aishun Jin

    doi:10.1101/2021.04.19.440481 Date: 2021-04-20 Source: bioRxiv

    Accumulating mutations in the SARS-CoV-2 Spike MESHD SARS-CoV-2 Spike PROTEIN ( S) protein PROTEIN can increase the possibility of immune escape, challenging the present COVID-19 MESHD prophylaxis and clinical interventions. Here, 3 receptor binding domain (RBD) specific monoclonal antibodies (mAbs), 58G6, 510A5 and 13G9, with high neutralizing potency blocking authentic SARS-CoV-2 virus displayed remarkable efficacy against authentic B.1.351 virus. Each of these 3 mAbs in combination with one neutralizing Ab recognizing non-competing epitope exhibited synergistic effect against authentic SARS-CoV-2 virus. Surprisingly, structural analysis revealed that 58G6 and 13G9, encoded by the IGHV1-58 HGNC and the IGKV3-20 germline genes, both recognized the steric region S470-495 on the RBD, overlapping the E484K mutation presented in B.1.351. Also, 58G6 directly bound to another region S450-458 in the RBD. Significantly, 58G6 and 510A5 both demonstrated prophylactic efficacy against authentic SARS-CoV-2 and B.1.351 viruses in the transgenic mice expressing human ACE2 HGNC ( hACE2 HGNC), protecting weight loss MESHD and reducing virus loads. These 2 ultrapotent neutralizing Abs can be promising candidates to fulfill the urgent needs for the prolonged COVID-19 pandemic MESHD.

    Ultrapotent SARS-CoV-2 neutralizing antibodies effectively block new-emerging mutational variants

    Authors: Aishun Jin; Tingting Li; Yingming Wang; Xiaojian Han; Chenjian Gu; Hangtian Guo; Kai Wang; Fengjiang Liu; Feiyang Luo; Jie Hu; Chao Hu; Shenglong Li; Yanan Hao; Jingjing Huang; Yingyi Long; Shuyi Song; Wang Wang; Meiying Shen; Ruixin Wu; Song Mu; Qian Chen; Fengxia Gao; Jianwei Wang; Shunhua Long; Luo Li; Yang Wu; Yan Gao; Wei Xu; Xia Cai; Di Qu; Qingzhu Gao; Guiji Zhang; Changlong He; Wei Wang; Xiaoyun Ji; Ni Tang; Zhenghong Yuan; Youhua Xie; Haitao Yang; Ai-Long Huang

    doi:10.21203/rs.3.rs-215131/v1 Date: 2021-02-06 Source: ResearchSquare

    Accumulating mutations on SARS-CoV-2 Spike PROTEIN SARS-CoV-2 Spike MESHD ( S) protein PROTEIN may increase the possibility of immune escape, challenging the present COVID-19 MESHD prophylaxis and clinical interventions. Here, in a panel of receptor binding domain (S-RBD) specific monoclonal antibodies (mAbs) with high neutralizing potency against authentic SARS-CoV-2, at least 6 of them were found to efficiently block the pseudovirus of 501Y.V2, a highly transmissible SARS-CoV-2 variant with escape mutations. The top 3 neutralizing Abs (13G9, 58G6 and 510A5) exhibited comparative ultrapotency as those being actively pursued for clinical development. Interestingly, the antigenic sites for the majority of our neutralizing Abs overlapped with a single epitope (13G9e) on S-RBD. Further, the 3-dimensional structures of 2 ultrapotent neutralizing Abs 13G9 or 58G6 in complex with SARS-CoV-2 S trimer demonstrated that both Abs bound to a steric region within S472–490. Moreover, a specific linear region (S450–457) was identified as an additional target for 58G6. Importantly, our cryo-electron microscopy (cryo-EM) analysis revealed a unique phenomenon that the S-RBDs interacting with the fragments of antigen binding (Fabs) of 13G9 or 58G6 encoded by the IGHV1-58 HGNC and the IGKV3-20 gene segments were universally in the ‘up’ conformation in all observed particles. The potent neutralizing Abs presented in the current study may be promising candidates to fulfill the urgent needs for the current pandemic of SARS-CoV-2, and may of fundamental value for the next-generation vaccine development.

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