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MeSH Disease

HGNC Genes

nibrin (2)


SARS-CoV-2 proteins

ORF1a (1)

ProteinN (1)


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    Potent neutralizing nanobodies resist convergent circulating variants of SARS-CoV-2 by targeting novel and conserved epitopes

    Authors: Dapeng Sun; Zhe Sang; Jeff Kim; Yufei Xiang; Tomer Cohen; Anna K Belford; Alexis Huet; James F Conway; Ji Sun; Derek Taylor; Cheng Zhang; Wei Huang; Yi Shi

    doi:10.1101/2021.03.09.434592 Date: 2021-03-10 Source: bioRxiv

    There is an urgent need to develop effective interventions resistant to the evolving variants of SARS-CoV-2. Nanobodies ( Nbs HGNC) are stable and cost-effective agents that can be delivered by novel aerosolization route to treat SARS-CoV-2 infections MESHD efficiently. However, it remains unknown if they possess broadly neutralizing activities against the prevalent circulating strains. We found that potent neutralizing Nbs HGNC are highly resistant to the convergent variants of concern that evade a large panel of neutralizing antibodies (Abs) and significantly reduce the activities of convalescent or vaccine-elicited sera. Subsequent determination of 9 high-resolution structures involving 6 potent neutralizing Nbs HGNC by cryoelectron microscopy reveals conserved and novel epitopes on virus spike inaccessible to Abs. Systematic structural comparison of neutralizing Abs and Nbs HGNC provides critical insights into how Nbs HGNC uniquely target the spike to achieve high-affinity and broadly neutralizing activity against the evolving virus. Our study will inform the rational design of novel pan-coronavirus vaccines and therapeutics.

    Reverse transcription loop-mediated isothermal amplification combined with nanoparticles-based biosensor for diagnosis of COVID-19 MESHD

    Authors: Xiong Zhu; Xiaoxia Wang; Limei Han; Ting Chen; Licheng Wang; Huan Li; Sha Li; Lvfen He; Xiaoying Fu; Shaojin Chen; Xing Mei; Hai Chen; Yi Wang

    doi:10.1101/2020.03.17.20037796 Date: 2020-03-20 Source: medRxiv

    Given the scale and rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, known as 2019-nCov) infection ( COVID-19 MESHD), the ongoing global SARS-CoV-2 outbreak has become a huge public health issue. Rapid and precise diagnostic methods are thus immediately needed for diagnosing COVID-19 MESHD, providing timely treatment and facilitating infection control. A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) coupled with nanoparticles-based biosensor ( NBS MESHD NBS HGNC) assay (RT-LAMP- NBS MESHD NBS HGNC) was successfully established for rapidly and accurately diagnosing COVID-19 MESHD. A simple equipment (such as heating block) was required for maintaining a constant temperature (63 C) for only 40 min. Using two designed LAMP primer sets, F1ab ( opening reading frame 1a PROTEIN/b) and np ( nucleoprotein PROTEIN) genes of SARS-CoV-2 were simultaneously amplified and detected in a one-step and single-tube reaction, and the detection results were easily interpreted by NBS MESHD NBS HGNC. The sensitivity of SARS-CoV-2 RT-LAMP-NBS MESHD NBS HGNC was 12 copies (each of detection target) per reaction, and no cross-reactivity was generated from non-SARS-CoV-2 templates. Among clinically diagnosed COVID-19 MESHD patients, the analytical sensitivity of SARS-CoV-2 was 100% (33/33) in the oropharynx swab samples, and the assay's specificity was also 100% (96/96) when analyzed the clinical samples collected from non- COVID-19 MESHD patients. The total diagnosis test from sample collection to result interpretation only takes approximately 1 h. In sum, the RT-LAMP- NBS MESHD NBS HGNC is a promising tool for diagnosing the current SARS-CoV-2 infection MESHD in first line field, public health and clinical laboratories, especially for resource-challenged regions.

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MeSH Disease
HGNC Genes
SARS-CoV-2 Proteins


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