Corpus overview


Overview

MeSH Disease

Human Phenotype

Transmission

There are no transmission terms in the subcorpus


Seroprevalence

There are no seroprevalence terms in the subcorpus

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    A Novel Purification Procedure for Active Recombinant Human DPP4 and the Inability of DPP4 to Bind SARS-CoV-2

    Authors: Cecy Xi; Arianna Arianna Di Fazio; Naveed Nadvi; Karishma Patel; Michelle Xiang; Hui Emma Zhang; Chandrika Deshpande; Jason Low; Xiaonan Trixie Wang; Yiqian Chen; Brenna Osborne; Ana Julia Vieira de Ribeiro; Geoffrey McCaughan; Bret Church; Joel Mackay; Mark Gorrell

    id:10.20944/preprints202009.0390.v1 Date: 2020-09-17 Source: Preprints.org

    Proteases catalyse irreversible posttranslational modifications that often alter a biological function of the substrate. The protease dipeptidyl peptidase 4 (DPP4) is a pharmacological target in type 2 diabetes MESHD therapy primarily because it inactivates glucagon-like protein-1. DPP4 also has roles in steatosis MESHD, insulin resistance HP, cancers MESHD and inflammatory and fibrotic diseases MESHD. In addition, DPP4 binds to the spike protein of MERS virus, causing it to be the human cell surface receptor for that virus. DPP4 has been identified as a potential binding target of SARS-CoV-2 spike protein, so this question requires experimental investigation. Understanding protein structure and function requires reliable protocols for production and purification. We developed such strategies for baculovirus generated soluble recombinant human DPP4 (residues 29-766) produced in insect cells. Purification used differential ammonium sulfate precipitation, hydrophobic interaction chromatography, dye affinity chromatography in series with immobilised metal affinity chromatography, and ion exchange chromatography. The binding affinities of DPP4 to the SARS-CoV-2 full-length spike protein and its receptor binding domain (RBD) were measured using surface plasmon resonance. This optimised DPP4 purification procedure yielded 1 to 1.8 mg of pure fully active soluble DPP4 protein per litre of insect cell culture with specific activity >30 U/mg, indicative of high purity. No specific binding between DPP4 and CoV-2 spike protein was detected. In summary, a procedure for high purity high yield soluble human DPP4 was achieved and used to show that, unlike MERS, SARS-CoV-2 does not bind human DPP4.

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MeSH Disease
Human Phenotype
Transmission
Seroprevalence


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