Corpus overview


Overview

MeSH Disease

Human Phenotype

Transmission

Seroprevalence
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    Serological Responses to Human Virome Define Clinical Outcomes of Italian Patients Infected with SARS-CoV-2 MESHD

    Authors: Limin Wang; Julian Candia; Lichun Ma; Yongmei Zhao; Luisa Imberti; Alessandra Sottini; Kerry Dobbs; - NIAID-NCI COVID Consortium; Andrea Lisco; Irini Sereti; Helen C. Su; Luigi D. Notarangelo; Xin Wei Wang; Junyoung Kim; Patricia S Lobo; Fabiolla S Santos; Alessandra AP Lima; Camila M Bragagnolo; Luana S Soares; Patricia SM Almeida; Darleise S Oliveira; Carolina KN Amorim; Iran B Costa; Dielle M Teixeira; Edvaldo T Penha Jr.; Delana AM Bezerra; Jones AM Siqueira; Fernando N Tavares; Felipe B Freitas; Janete TN Rodrigues; Janaina Mazaro; Andreia S Costa; Marcia SP Cavalcante; Marineide Souza Silva; Ilvanete A Silva; Gleissy AL Borges; Lidio G Lima; Hivylla LS Ferreira; Miriam TFP Livorati; Andre L Abreu; Arnaldo C Medeiros; Hugo R Resque; Rita CM Sousa; Giselle MR Viana

    doi:10.1101/2020.09.04.20187088 Date: 2020-09-07 Source: medRxiv

    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the pandemic respiratory infectious disease MESHD COVID-19. However, clinical manifestations and outcomes differ significantly among COVID-19 patients, ranging from asymptomatic TRANS to extremely severe, and it remains unclear what drives these disparities. Here, we studied 159 hospitalized Italian patients with pneumonia HP pneumonia MESHD from the NIAID-NCI COVID-19 Consortium using a phage-display method to characterize circulating antibodies SERO binding to 93,904 viral peptides encoded by 1,276 strains of human viruses. SARS-CoV-2 infection MESHD was associated with a marked increase in individual's immune memory antibody SERO repertoires linked to trajectories of disease severity from the longitudinal analysis also including anti-spike protein antibodies SERO. By applying a machine-learning-based strategy, we developed a viral exposure signature predictive of COVID-19-related disease severity linked to patient survival. These results provide a basis for understanding the roles of memory B-cell repertoires in COVID-19-related symptoms as well as a predictive tool for monitoring its clinical severity.

    Seroprevalence SERO of anti- SARS-CoV-2 antibodies SERO in COVID-19 patients and healthy volunteers

    Authors: Patricia Figueiredo-Campos; Birte Blankenhaus; Catarina Mota; Andreia Gomes; Marta Serrano; Silvia Ariotti; Catarina Costa; Helena Nunes-Cabaco; Antonio M Mendes; Pedro Gaspar; Conceicao M Pereira-Santos; Fabiana Rodrigues; Jorge Condeco; Antonia M Escoval; Matilde Santos; Mario Ramirez; Jose Melo-Cristino; Pedro J Simas; Eugenia Vasconcelos; Angela Afonso; Marc Veldhoen; Matthew Harnett; Melody Eaton; Sandra Hatem; Hajra Jamal; Alara Akyatan; Alexandra Tabachnikova; Lora E. Liharska; Liam Cotter; Brian Fennessey; Akhil Vaid; Guillermo Barturen; Scott R. Tyler; Hardik Shah; Yinh-chih Wang; Shwetha Hara Sridhar; Juan Soto; Swaroop Bose; Kent Madrid; Ethan Ellis; Elyze Merzier; Konstantinos Vlachos; Nataly Fishman; Manying Tin; Melissa Smith; Hui Xie; Manishkumar Patel; Kimberly Argueta; Jocelyn Harris; Neha Karekar; Craig Batchelor; Jose Lacunza; Mahlet Yishak; Kevin Tuballes; Leisha Scott; Arvind Kumar; Suraj Jaladanki; Ryan Thompson; Evan Clark; Bojan Losic; - The Mount Sinai COVID-19 Biobank Team; Jun Zhu; Wenhui Wang; Andrew Kasarskis; Benjamin S. Glicksberg; Girish Nadkarni; Dusan Bogunovic; Cordelia Elaiho; Sandeep Gangadharan; George Ofori-Amanfo; Kasey Alesso-Carra; Kenan Onel; Karen M. Wilson; Carmen Argmann; Marta E. Alarcón-Riquelme; Thomas U. Marron; Adeeb Rahman; Seunghee Kim-Schulze; Sacha Gnjatic; Bruce D. Gelb; Miriam Merad; Robert Sebra; Eric E. Schadt; Alexander W. Charney

    doi:10.1101/2020.08.30.20184309 Date: 2020-09-02 Source: medRxiv

    SARS-CoV-2 has emerged as a novel human pathogen, causing clinical signs, from fever HP fever MESHD to pneumonia HP pneumonia MESHD - COVID-19 - but may remain mild or even asymptomatic TRANS. To understand the continuing spread of the virus, to detect those who are and were infected, and to follow the immune response longitudinally, reliable and robust assays for SARS-CoV-2 detection and immunological monitoring are needed and have been setup around the world. We quantified immunoglobulin M (IgM), IgG and IgA antibodies SERO recognizing the SARS-CoV-2 receptor-binding domain (RBD) or the Spike (S) protein over a period of five months following COVID-19 disease onset or in previously SARS-CoV-2 PCR-positive volunteers. We report the detailed setup to monitor the humoral immune response from over 300 COVID-19 hospital patients and healthcare workers, 2500 University staff and 187 post-COVID19 volunteers, and assessing titres for IgM, IgG and IgA. Anti- SARS-CoV-2 antibody SERO responses followed a classic pattern with a rapid increase within the first three weeks after symptoms. Although titres reduce from approximately four weeks, the ability to detect SARS-CoV-2 antibodies SERO remained robust for five months in a large proportion of previously virus-positive screened subjects. Our work provides detailed information for the assays used, facilitating further and longitudinal analysis of protective immunity to SARS-CoV-2. Moreover, it highlights a continued level of circulating neutralising antibodies SERO in most people with confirmed SARS-CoV-2, at least up to five months after infection.

    Clinical Characteristics of Severe Covid Pneumonia HP: Exploring New Trends in ICU

    Authors: Aftab Akhtar; Sheher Bano; Ahtesham Iqbal; Moazma Ramzan; Aayesha Qadeer; Syed Waqar Hussain; Anam Saleem; Omair-ul-Haq Lodhi; Sana Zubair; Abdul Hameed kiani

    doi:10.21203/rs.3.rs-69224/v1 Date: 2020-08-31 Source: ResearchSquare

    Background: In late December 2019, Covid-19 emerged as clusters of pneumonia HP pneumonia MESHD of unknown cause in a province of china, Wuhan. Etiological agent was identified as novel coronavirus that resembles severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East Respiratory syndrome coronavirus (MERS-CoV) MESHD and has zoonotic transmission TRANS. Covid pneumonia HP can remain asymptomatic TRANS, present as mild infection, severe HP infection, severe MESHD pneumonia HP pneumonia MESHD or respiratory failure HP respiratory failure MESHD. Diagnosis is based on rRT-PCR carried out on respiratory secretions. Covid related mortality exceeds 50% once patient requires ICU admission. Objective: To study the characteristics of ICU population admitted to ICU of Shifa International hospital.Results: we prospectively analysed 74 patients which included 43.3% females TRANS and 56.7% males TRANS. Commonest symptoms were shortness of breath MESHD (94.5%), fever HP fever MESHD (74.3%) and cough HP (74.3%). Most of our study population consisted of non-smokers (79.7%) and had hypertension HP hypertension MESHD (59.4%) followed by diabetes MESHD (47.2%). Hydroxychloroquine (HCQ) and azithromycin combination is superior to hydroxychloroquine and doxycycline in reducing mortality (p=0.023) whereas Doxycycline alone resulted in increased mortality (p=0.009). Those who did not require antibiotics or required only narrow spectrum antibiotics had increased survival and reduced requirement of invasive mechanical ventilation (p=< 0.0001). in our study population, (44.9%) developed acute kidney injury HP acute kidney injury MESHD, 2.7% needed re-intubations 10.8% developed surgical emphysema HP emphysema MESHD and 2.7 % thromboembolic MESHD events despite full anticoagulation. ICU mortality was 41.8% and was higher in females TRANS (59.4%, p=0.008), those who had SOFA score > 3.5 at time of admission, raised D-Dimers > 931 ng/ml, NLR > 9.2. It was further high in those who required invasive mechanical ventilation and vasopressor support (58.1% mortality p=< 0.001). ICU stay was more prolonged in those requiring invasive mechanical ventilation as compared to those who did not. (23 days vs 6 days, p=0.001). Mean plateau pressure was 19.6 ± 7.6; mean Driving pressures 14.4 ± 4.6; mean PaO2/FiO2 150.7 ± 73.9; mean SPO2/FiO2 173.9 ± 106.9; mean PEEP was 8.2 ±4.33.Conclusion: We concluded that severe covid pneumonia HP pneumonia MESHD is common amongst males TRANS, non-smokers those who had comorbid. HCQ and azithromycin combination is superior to combination of HCQ and doxycycline or doxycycline alone and QT prolongation MESHD is a rare complication. Baseline NLR, APACHI II, SOFA, SAPS II, NUTRIC scores, D-Dimers, invasive ventilation and vasopressor support are important tools to predict ICU mortality. Invasive mechanical ventilation carries higher mortality and associated with more prolonged ICU stay. AKI is most common complication followed by shock HP and surgical emphysema HP emphysema MESHD. CRP, Ferritin levels has no impact on outcome.

    Preoperative Computerized Tomography Screening for COVID-19 Pneumonia HP in Asymptomatic TRANS Patients: experiences from two centers

    Authors: Terman Gumus; Zeynep Unal Kabaoglu; Furkan Kartal; Bilgen Coskun; Feyzi Artukoglu; K. Cetin Atasoy

    doi:10.21203/rs.3.rs-63660/v1 Date: 2020-08-21 Source: ResearchSquare

    BACKGROUND: Chest CT screening for COVID-19 in preoperative patients is recommended in selected patients. The aim of this retrospective study is to evaluate the preoperative screening performance SERO of chest CT (computerized tomography) examination to detect COVID-19 positive individuals. STUDY DESIGN: In this retrospective study 218 adult TRANS patients who had preoperative chest CT and RT-PCR were enrolled. CT imaging results, which have been reported according to the Radiological Society of North America (RSNA) expert consensus on COVID-19, were collected from the picture archiving and communicating system (PACS). Demographic data, planned surgeries, and postoperative outcomes were collected from the electronic patient records.RESULTS: One patient (0.5%) showed typical CT features for COVID-19 pneumonia HP pneumonia MESHD; 12 patients (5.5%) were reported as indeterminate, and eight (3.7%) were reported as atypical for COVID-19 pneumonia HP pneumonia MESHD. Only one of the three patients with positive RT-PCR had abnormalities on CT. When RT-PCR tests were taken as reference, the sensitivity SERO, specificity, and accuracy of chest CT in showing COVID-19 infection MESHD in asymptomatic TRANS patients were 33.3%, 90.7%, and 90.0% respectively.CONCLUSION: Chest CT screening for COVID-19 has a very low yield in asymptomatic TRANS preoperative patients and shows false positive findings in 9.2% of cases, potentially leading to unnecessary postponing of the surgery. 

    COVID-19: Exhaled virus detected by Face Mask Sampling provides new links to disease severity and potential infectivity.

    Authors: Caroline Williams; Daniel Pan; Jonathan Decker; Anika Wisniewska; Shirley Sze; Eve Fletcher; Richard Haigh; Mohamad Abdulwhhab; Paul Bird; Christopher Holmes; Alaa Al-Taie; Baber Saleem; Jingzhe Pan; Natalie Garton; Manish Pareek; Michael Barer; Craig J Smith; Stuart M Allan; Michelle M. Lister; Hannah C. Howson-Wells; Edward C Holmes; Matthew W. Loose; Jonathan K. Ball; C. Patrick McClure; - The COVID-19 Genomics UK consortium study group; Shi Chen

    doi:10.1101/2020.08.18.20176693 Date: 2020-08-21 Source: medRxiv

    Background Nasopharyngeal samples (NPS) are the mainstay of COVID-19 diagnosis. However, the extent to which assay signals relate to exhaled virus is unknown. We investigated the use of novel, non-invasive face-mask sampling (FMS) to detect exhaled SARS-CoV-2 RNA in two studies. Methods In an outbreak study (cohort 1), we performed FMS MESHD and NPS for 21 consecutive days after diagnosis on six healthcare workers who were screened positive for SARS-CoV-2. In a second hospitalised cohort (cohort 2), we performed FMS on 47 patients within 24 hours of a positive diagnosis. COVID-19 severity was graded according to WHO recommendations. Findings In cohort 1, SARS-COV-2 was detected by FMS in 10/40 (25%) samples (4/6 individuals), with no correlation between NPS and FMS RNA signals. All samples were negative by day 14 post diagnosis. Sustained FMS positivity with higher viral RNA signals showed a trend towards disease severity. In cohort 2, 19/47 (40%) individuals exhaled SARS-CoV-2 RNA extending over five orders of magnitude. FMS positive participants were older (positive: median age TRANS [IQR] 71 [61-84] vs negative: 61 [45-73], p=0.04) with more comorbidities (positive: 2 [1-3] vs negative: 1 [0-2], p<0.001) and have active cough HP (positive: 68% vs negative: 24%, p=0.003) and breathlessness (positive: 74% vs negative: 32%, p=0.005) during sampling, compared to FMS negative patients. Of five patients who were FMS positive and asymptomatic TRANS at time of sampling, two died of severe COVID-19 pneumonia HP pneumonia MESHD within one month of follow up. Interpretation FMS detects exhaled SARS-COV-2, with stronger signals in those who develop severe disease.

    Transmission TRANS Dynamics of SARS-CoV-2 in a Mid-size City of China

    Authors: Hongjun Zhao; Xiaoxiao Lu; Wenhui Lun; Tiegang Li; Boqi Rao; Dedong Wang; Di Wu; Fuman Qiu; Zhicong Yang; Lu Jiachun

    doi:10.21203/rs.3.rs-59402/v1 Date: 2020-08-14 Source: ResearchSquare

    Background: An outbreak of pneumonia HP pneumonia MESHD associated with the severe acute respiratory syndrome coronavirus 2 MESHD (SARS-CoV-2) emerged in Wuhan city and then spread to other cities. It is very urgent to delineate the epidemiological and clinical characteristics of these affected patients. Methods: To investigate the epidemiological characteristics of the COVID-19, we describe a case series of 473 patients with confirmed COVID-19 in Wenzhou of China from January 27 to March 2, 2020.Results: The median age TRANS of all patients was 47.6 years, 48.4% of which were female TRANS. 33.8% of the patients had a history of residence in Wuhan. Fever HP Fever MESHD (71.7%) and cough HP (43.1%) were the most common symptoms. In addition, three kinds of unconventional cases were observed, namely 4.9% asymptomatic TRANS patients, 7.6% confirmed patients who had no link to Wuhan city but contact with individuals from Wuhan without any symptoms at the time of contact, and 12.9% confirmed patients who had an unknown source of transmission TRANS. We estimated that the basic reproductive number TRANS ( R0 TRANS) was 2.75 (95%CI: 2.37-3.23). The effective reproduction number TRANS (Rt) fluctuated within the range of 2.50 to 3.74 from January 11 to January 16 while gradually reached the peak of 3.74 on January 16. Rt gradually decreased after January 16 and decreased to 1.00 on January 30. Rt continually decreased and reached the lowest point (0.03) on February 21, 2020.Conclusion: Our findings presented the possibility of asymptomatic TRANS carriers TRANS affected with SARS-CoV-2, and this phenomenon suggested that chances of uncontrollable transmission TRANS in the larger population might be higher than formerly estimated, and transmission TRANS by these three kinds of unconventional patients in Wenzhou may be an important characteristic of infection MESHD in other mid-sized cities in the world. This study evaluated the epidemic characteristics of Wenzhou after having cases imported from Hubei Province and the effects after adopting a series of strict prevention and control strategy. 

    A Large-Scale Clinical Validation Study Using nCapp Cloud Plus Terminal by Frontline Doctors for the Rapid Diagnosis of COVID-19 and COVID-19 pneumonia HP pneumonia MESHD in China

    Authors: Dawei Yang; Tao Xu; Xun Wang; Deng Chen; Ziqiang Zhang; Lichuan Zhang; Jie Liu; Kui Xiao; Li Bai; Yong Zhang; Lin Zhao; Lin Tong; Chaomin Wu; Yaoli Wang; Chunling Dong; Maosong Ye; Yu Xu; Zhenju Song; Hong Chen; Jing Li; Jiwei Wang; Fei Tan; Hai Yu; Jian Zhou; Jinming Yu; Chunhua Du; Hongqing Zhao; Yu Shang; Linian Huang; Jianping Zhao; Yang Jin; Charles A. Powell; Yuanlin Song; Chunxue Bai

    doi:10.1101/2020.08.07.20163402 Date: 2020-08-11 Source: medRxiv

    Background The outbreak of coronavirus disease MESHD 2019 (COVID-19) has become a global pandemic acute infectious disease MESHD, especially with the features of possible asymptomatic TRANS carriers TRANS and high contagiousness. It causes acute respiratory distress HP respiratory distress MESHD syndrome and results in a high mortality rate if pneumonia HP is involved. Currently, it is difficult to quickly identify asymptomatic TRANS cases or COVID-19 patients with pneumonia HP pneumonia MESHD due to limited access to reverse transcription-polymerase chain reaction (RT-PCR) nucleic acid tests and CT scans, which facilitates the spread of the disease TRANS at the community level, and contributes to the overwhelming of medical resources in intensive care units. Goal This study aimed to develop a scientific and rigorous clinical diagnostic tool for the rapid prediction of COVID-19 cases based on a COVID-19 clinical case database in China, and to assist global frontline doctors to efficiently and precisely diagnose asymptomatic TRANS COVID-19 patients and cases who had a false-negative RT-PCR test result. Methods With online consent, and the approval of the ethics committee of Zhongshan Hospital Fudan Unversity (approval number B2020-032R) to ensure that patient privacy is protected, clinical information has been uploaded in real-time through the New Coronavirus Intelligent Auto-diagnostic Assistant Application of cloud plus terminal (nCapp) by doctors from different cities (Wuhan, Shanghai, Harbin, Dalian, Wuxi, Qingdao, Rizhao, and Bengbu) during the COVID-19 outbreak in China. By quality control and data anonymization on the platform, a total of 3,249 cases from COVID-19 high-risk groups were collected. These patients had SARS-CoV-2 RT-PCR test results and chest CT scans, both of which were used as the gold standard for the diagnosis of COVID-19 and COVID-19 pneumonia HP pneumonia MESHD. In particular, the dataset included 137 indeterminate cases who initially did not have RT-PCR tests and subsequently had positive RT-PCR results, 62 suspected cases who initially had false-negative RT-PCR test results and subsequently had positive RT-PCR results, and 122 asymptomatic TRANS cases who had positive RT-PCR test results, amongst whom 31 cases were diagnosed. We also integrated the function of a survey in nCapp to collect user feedback from frontline doctors. Findings We applied the statistical method of a multi-factor regression model to the training dataset (1,624 cases) and developed a prediction model for COVID-19 with 9 clinical indicators that are fast and accessible: 'Residing or visiting history in epidemic regions', 'Exposure history to COVID-19 patient', 'Dry cough HP', ' Fatigue HP', 'Breathlessness', 'No body temperature decrease after antibiotic treatment', 'Fingertip blood SERO oxygen saturation<=93%', ' Lymphopenia HP Lymphopenia MESHD', and 'C-reactive protein (CRP) increased'. The area under the receiver operating characteristic (ROC) curve (AUC) for the model was 0.88 (95% CI: 0.86, 0.89) in the training dataset and 0.84 (95% CI: 0.82, 0.86) in the validation dataset (1,625 cases). To ensure the sensitivity SERO of the model, we used a cutoff value of 0.09. The sensitivity SERO and specificity of the model were 98.0% (95% CI: 96.9%, 99.1%) and 17.3% (95% CI: 15.0%, 19.6%), respectively, in the training dataset, and 96.5% (95% CI: 95.1%, 98.0%) and 18.8% (95% CI: 16.4%, 21.2%), respectively, in the validation dataset. In the subset of the 137 indeterminate cases who initially did not have RT-PCR tests and subsequently had positive RT-PCR results, the model predicted 132 cases, accounting for 96.4% (95% CI: 91.7%, 98.8%) of the cases. In the subset of the 62 suspected cases who initially had false-negative RT-PCR test results and subsequently had positive RT-PCR results, the model predicted 59 cases, accounting for 95.2% (95% CI: 86.5%, 99.0%) of the cases. Considering the specificity of the model, we used a cutoff value of 0.32. The sensitivity SERO and specificity of the model were 83.5% (95% CI: 80.5%, 86.4%) and 83.2% (95% CI: 80.9%, 85.5%), respectively, in the training dataset, and 79.6% (95% CI: 76.4%, 82.8%) and 81.3% (95% CI: 78.9%, 83.7%), respectively, in the validation dataset, which is very close to the published AI model. The results of the online survey 'Questionnaire Star' showed that 90.9% of nCapp users in WeChat mini programs were 'satisfied' or 'very satisfied' with the tool. The WeChat mini program received a significantly higher satisfaction rate than other platforms, especially for 'availability and sharing convenience of the App' and 'fast speed of log-in and data entry'. Discussion With the assistance of nCapp, a mobile-based diagnostic tool developed from a large database that we collected from COVID-19 high-risk groups in China, frontline doctors can rapidly identify asymptomatic TRANS patients and avoid misdiagnoses of cases with false-negative RT-PCR results. These patients require timely isolation or close medical supervision. By applying the model, medical resources can be allocated more reasonably, and missed diagnoses can be reduced. In addition, further education and interaction among medical professionals can improve the diagnostic efficiency for COVID-19, thus avoiding the transmission TRANS of the disease from asymptomatic TRANS patients at the community level.

    K18-hACE2 Mice for Studies of COVID-19 Treatments and Pathogenesis Including Anosmia HP

    Authors: Stanley Perlman; Jian Zheng; LOK YIN ROY WONG; Kun Li; Abhishek K Verma; Miguel E Ortiz Bezara; Christine Wohlford-Lenane; Mariah R. Leidinger; Michael C. Kundson; David K. Meyerholz; Paul B McCray Jr.

    doi:10.1101/2020.08.07.242073 Date: 2020-08-10 Source: bioRxiv

    The ongoing COVID-19 pandemic is associated with substantial morbidity and mortality. While much has been learned in the first months of the pandemic, many features of COVID-19 pathogenesis remain to be determined. For example, anosmia HP anosmia MESHD is a common presentation and many patients with this finding show no or only minor respiratory signs. Studies in animals experimentally infected with SARS-CoV-2, the cause of COVID-19, provide opportunities to study aspects of the disease not easily investigated in human patients. COVID-19 severity ranges from asymptomatic TRANS to lethal. Most experimental infections provide insights into mild disease. Here, using K18-hACE2 mice that we originally developed for SARS studies, we show that infection with SARS-CoV-2 causes severe disease in the lung, and in some mice, the brain. Evidence of thrombosis MESHD and vasculitis HP vasculitis MESHD was detected in mice with severe pneumonia HP pneumonia MESHD. Further, we show that infusion of convalescent plasma SERO (CP) from a recovered COVID-19 patient provided protection against lethal disease. Mice developed anosmia HP anosmia MESHD at early times after infection MESHD. Notably, while treatment with CP MESHD prevented significant clinical disease, it did not prevent anosmia HP anosmia MESHD. Thus K18-hACE2 mice provide a useful model for studying the pathological underpinnings of both mild and lethal COVID-19 and for assessing therapeutic interventions.

    Viral shedding and immunological features of children TRANS COVID-19 patients

    Authors: Yang Yang; Haixia Zheng; Ling Peng; Jinli Wei; Yanrong Wang; Hexiao Li; Bo Peng; Shisong Fang; Mingxia Zhang; Yanjie Li; Hui Liu; Kai Feng; Li Xing; Jun Wang; Mengli Cao; Fuxiang Wang; Lei Liu; Yingxia Liu; Jing Yuan

    doi:10.21203/rs.3.rs-48544/v1 Date: 2020-07-24 Source: ResearchSquare

    Background SARS-CoV-2 could infect people at all ages TRANS, and the viral shedding and immunological features of children TRANS COVID-19 patients were analyzed.Methods Epidemiological information and clinical data were collected from 35 children TRANS patients. Viral RNAs in respiratory and fecal samples were detected. Plasma SERO of 11 patients were collected and measured for 48 cytokines.Results 40% (14/35) of the children TRANS COVID-19 patients showed asymptomatic TRANS infections MESHD, while pneumonia HP pneumonia MESHD shown by CT scan occurred in most of the cases (32/35, 91.43%). Elevated LDH, AST, CRP, neutropenia HP neutropenia MESHD, leukopenia HP leukopenia MESHD, lymphopenia HP lymphopenia MESHD and thrombocytopenia HP thrombocytopenia MESHD occurred in some cases, and CD4 and CD8 counts were normal. A total of 22 cytokines were significantly higher than the healthy control, and IP-10, IFN-α2 of them in children TRANS were significantly lower than the adult TRANS patients. Meanwhile, MCP-3, HGF, MIP-1α, and IL-1ra were similar or lower than healthy control, while significantly lower than adult TRANS patients. Viral RNAs were detected as early as the first day after illness onset (d.a.o) in both the respiratory and fecal samples. Viral RNAs decreased as the disease progression and mostly became negative in respiratory samples within 18 d.a.o, while maintained relatively stable during the disease progression and still detectable in some cases during 36~42 d.a.o. Conclusion COVID-19 in children TRANS was mild, and asymptomatic TRANS infection MESHD was common. Immune responses were relatively normal in children TRANS COVID-19 patients. Cytokine storm also occurred in children TRANS patients, while much weaker than adult TRANS patients. Positive rate of viral RNAs in fecal samples was high, and profile of viral shedding were different between respiratory and gastrointestinal tract.

    Viral shedding and immunological features of children TRANS COVID-19 patients

    Authors: Yang Yang; Haixia Zheng; Ling Peng; Jinli Wei; Yanrong Wang; Hexiao Li; Bo Peng; Shisong Fang; Mingxia Zhang; Yanjie Li; Hui Liu; Kai Feng; Li Xing; Jun Wang; Mengli Cao; Fuxiang Wang; Lei Liu; Yingxia Liu; Jing Yuan

    doi:10.21203/rs.3.rs-48544/v2 Date: 2020-07-24 Source: ResearchSquare

    Background SARS-CoV-2 could infect people at all ages TRANS, and the viral shedding and immunological features of children TRANS COVID-19 patients were analyzed.Methods Epidemiological information and clinical data were collected from 35 children TRANS patients. Viral RNAs in respiratory and fecal samples were detected. Plasma SERO of 11 patients were collected and measured for 48 cytokines.Results 40% (14/35) of the children TRANS COVID-19 patients showed asymptomatic TRANS infections MESHD, while pneumonia HP pneumonia MESHD shown by CT scan occurred in most of the cases (32/35, 91.43%). Elevated LDH, AST, CRP, neutropenia HP neutropenia MESHD, leukopenia HP leukopenia MESHD, lymphopenia HP lymphopenia MESHD and thrombocytopenia HP thrombocytopenia MESHD occurred in some cases, and CD4 and CD8 counts were normal. A total of 22 cytokines were significantly higher than the healthy control, and IP-10, IFN-α2 of them in children TRANS were significantly lower than the adult TRANS patients. Meanwhile, MCP-3, HGF, MIP-1α, and IL-1ra were similar or lower than healthy control, while significantly lower than adult TRANS patients. Viral RNAs were detected as early as the first day after illness onset (d.a.o) in both the respiratory and fecal samples. Viral RNAs decreased as the disease progression and mostly became negative in respiratory samples within 18 d.a.o, while maintained relatively stable during the disease progression and still detectable in some cases during 36~42 d.a.o. Conclusion COVID-19 in children TRANS was mild, and asymptomatic TRANS infection MESHD was common. Immune responses were relatively normal in children TRANS COVID-19 patients. Cytokine storm also occurred in children TRANS patients, while much weaker than adult TRANS patients. Positive rate of viral RNAs in fecal samples was high, and profile of viral shedding were different between respiratory and gastrointestinal tract.

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MeSH Disease
Human Phenotype
Transmission
Seroprevalence


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