Corpus overview


MeSH Disease

Human Phenotype


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    Efficient production of Moloney murine leukemia HP leukemia MESHD virus-like particles pseudotyped with the severe acute respiratory syndrome coronavirus-2 MESHD (SARS-CoV-2) spike protein

    Authors: Manuel Caruso; Sylvie Roy; Karim Ghani; Pedro Otavio de Campos-Lima; Leonard A. Daly; Fabrice Agou; Claire E. Eyers; Patrick A. Eyers; Marco Vignuzzi; Edward Emmott; Fritz Soergel

    doi:10.1101/2020.09.16.298992 Date: 2020-09-16 Source: bioRxiv

    The severe acute respiratory syndrome coronavirus 2 MESHD (SARS-CoV-2) outbreak that started in China at the end of 2019 has rapidly spread to become pandemic. Several investigational vaccines that have already been tested in animals and humans were able to induce neutralizing antibodies SERO against the SARS-CoV-2 spike MESHD (S) protein, however protection and long-term efficacy in humans remain to be demonstrated. We have investigated if a virus-like particle (VLP) derived from Moloney murine leukemia HP leukemia MESHD virus ( MLV MESHD) could be engineered to become a candidate SARS-CoV-2 vaccine amenable to mass production. First, we showed that a codon optimized version of the S protein could migrate efficiently to the cell membrane. However, efficient production of infectious nanoparticles was only achieved with stable expression of a shorter version of S in its C-terminal domain (DeltaS) in 293 cells that express MLV Gag-Pol (293GP). The incorporation of DeltaS was 15-times more efficient into VLPs as compared to the full-length version, and that was not due to steric interference between the S cytoplasmic tail and the MLV capsid. Indeed, a similar result was also observed with extracellular vesicles released from parental 293 cells. The amount of DeltaS incorporated into VLPs released from producer cells was robust, with an estimated 1.25 microg/ml S2 equivalent (S is comprised of S1 and S2). Thus, a scalable platform that has the potential for production of pan-coronavirus VLP vaccines is described. The resulting nanoparticles could potentially be used alone or as a boost for other immunization strategies for COVID-19.

    Pathological Study of the 2019 Novel Coronavirus Disease MESHD (COVID-19) through Post-Mortem Core Biopsies

    Authors: Sufang Tian; Yong Xiong; Huan Liu; Li Niu; Jianchun Guo; Meiyan Liao; Shu-Yuan Xiao

    id:10.20944/preprints202003.0311.v1 Date: 2020-03-20 Source:

    Data on pathologic changes of the 2019 novel coronavirus disease MESHD (COVID-19) are scarce. To gain knowledge about the pathology that may contribute to disease progression and fatality, we performed post-mortem needle core biopsies of lung, liver, and heart in four patients who died of COVID-19 pneumonia HP pneumonia MESHD. The patients’ ages TRANS ranged from 59 to 81, including 3 males TRANS and 1 female TRANS. Each patient had at least one underlying disease, including immunocompromised status ( chronic lymphocytic leukemia MESHD leukemia HP and renal transplantation) or other conditions ( cirrhosis HP cirrhosis MESHD, hypertension HP hypertension MESHD, and diabetes MESHD). Time from disease onset to death ranged from 15 to 52 days. All patients had elevated white blood SERO cell counts, with significant rise toward the end, and all had lymphocytopenia MESHD except for the patient with leukemia HP leukemia MESHD. Histologically, the main findings are in the lungs, including injury to the alveolar MESHD epithelial cells, hyaline membrane formation, and hyperplasia of type II pneumocytes MESHD, all components of diffuse alveolar damage MESHD. Consolidation by fibroblastic proliferation with extracellular matrix and fibrin forming clusters in airspaces is evident. In one patient, the consolidation consists of abundant intra-alveolar neutrophilic infiltration MESHD, consistent with superimposed bacterial bronchopneumonia MESHD. The liver exhibits mild lobular infiltration by small lymphocytes, and centrilobular sinusoidal dilation. Patchy necrosis is also seen. The heart shows only focal mild fibrosis MESHD and mild myocardial hypertrophy MESHD, changes likely related to the underlying conditions. In conclusion, the post-mortem examinations show advanced diffuse alveolar damage MESHD, as well as superimposed bacterial pneumonia HP pneumonia MESHD in some patients. Changes in the liver and heart are likely secondary or related to the underlying diseases.

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MeSH Disease
Human Phenotype

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