Corpus overview


Overview

MeSH Disease

Human Phenotype

Transmission

Seroprevalence

There are no seroprevalence terms in the subcorpus

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    Evidence of SARS-CoV-2 transcriptional activity in cardiomyocytes of COVID-19 patients without clinical signs of cardiac involvement

    Authors: Gaetano Pietro Bulfamante; Gianluca Lorenzo Perrucci; Monica Falleni; Elena Sommariva; Delfina Tosi; Carla Martinelli; Paola Songia; Paolo Poggio; Stefano Carugo; Giulio Pompilio

    doi:10.1101/2020.08.24.20170175 Date: 2020-08-26 Source: medRxiv

    Background - Cardiovascular complication in patients affected by novel Coronavirus respiratory disease MESHD (COVID-19) are increasingly recognized. However, although a cardiac tropism of SARS-CoV-2 MESHD for inflammatory cells in autopsy heart samples of COVID-19 patients has been reported, the presence of the virus in cardiomyocytes has not been documented yet. Methods - We investigated for SARS-CoV-2 presence in heart tissue autopsies of 6 consecutive COVID-19 patients deceased for respiratory failure HP respiratory failure MESHD showing no signs of cardiac involvement MESHD and with no history of heart disease MESHD. Cardiac autopsy samples were analysed by digital PCR, Western blot, immunohistochemistry, immunofluorescence, RNAScope, and transmission TRANS electron microscopy assays. Results - The presence of SARS-CoV-2 into cardiomyocytes was invariably detected. A variable pattern of cardiomyocytes injury MESHD was observed, spanning from the absence of cell death and subcellular alterations hallmarks to the intracellular oedema MESHD and sarcomere ruptures MESHD. In addition, we found active viral transcription in cardiomyocytes, by detecting both sense and antisense SARS-CoV-2 spike RNA. Conclusions - In this analysis of autopsy cases, the presence of SARS-CoV-2 into cardiomyocytes, determining variable patterns of intracellular involvement, has been documented. All these findings suggest the need of a cardiologic surveillance even in survived COVID-19 patients not displaying a cardiac phenotype, in order to monitor potential long-term cardiac sequelae.

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MeSH Disease
Human Phenotype
Transmission
Seroprevalence


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