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MeSH Disease

Human Phenotype

Transmission

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Seroprevalence
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    SARS-CoV-2 transcriptome analysis and molecular cataloguing of immunodominant epitopes for multi-epitope based vaccine design

    Authors: Sandeep Kumar Kushwaha Sr.; Veerbhan Kesarwani Jr.; Samraggi Choudhury Jr.; Sonu Gandhi Sr.; Shailesh Sharma Sr.

    doi:10.1101/2020.05.14.097170 Date: 2020-05-15 Source: bioRxiv

    SARS-CoV-2 is a single-stranded RNA virus that has caused more than 0.29 million deaths worldwide as of May 2020, and influence of COVID-19 pandemic is increasing continuously in the absence of approved vaccine and drug. Moreover, very limited information is available about SARS-CoV-2 expressed regions and immune responses. In this paper an effort has been made, to facilitate vaccine development by proposing multiple epitopes as potential vaccine candidates by utilising SARS-CoV-2 transcriptome data. Here, publicly available RNA-seq data of SARS-CoV-2 infection MESHD in NHBE and A549 human cell lines were used to construct SARS-CoV-2 transcriptome to understand disease pathogenesis and immune responses. In the first step, epitope prediction, MHC class I and II gene identification for epitopes, population coverage, antigenicity, immunogenicity, conservation and crossreactivity analysis with host antigens were performed by using SARS-CoV-2 transcriptome, and in the second step, structural compatibility of identified T-and B-cell epitopes were evaluated with MHC molecules and B-cell receptors through molecular docking studies. Quantification of MHC gene expression was also performed that indicated high variation in allele types and expression level of MHC genes with respect to cell lines. In A549 cell line, HLA-A*30:01:01:01 and HLA-B*44:03:01:01 were highly expressed, whereas 92 variants of HLA-A*24 genes such as HLA-A*24:02:01:01, HLA-A*24:286, HLA-A*24:479Q, HLA-A*24:02:134 and HLA-A*24:02:116 were highly expressed in NHBE cell lines. Prevalence SERO of HLA-A*24 alleles was suggested as risk factors for H1N1 infection MESHD, and associated with type-1 diabetes MESHD. HLA-C*03:03, linked with male infertility HP male infertility TRANS male infertility MESHD factors was also highly expressed in SARS-CoV-2 infected MESHD NHBE cell lines. Finally, three potential T-cell and five B-cell epitopes were selected for molecular docking studies with twenty-two MHC molecules and two B-cell receptors respectively. The results of in silico analysis indicated that proposed epitopes have high potential to recognize immune response of SARS-CoV-2 infection MESHD. This study will facilitate in vitro and in vivo vaccine related research studies.

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MeSH Disease
Human Phenotype
Transmission
Seroprevalence


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