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MeSH Disease

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SARS-CoV-2 proteins

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    Decreased neutrophil phagocytosis MESHD and killing of bacteria in COVID-19 MESHD patients

    Authors: Masoumeh Nomani; Mohammad Varahram; Payam Tabarsi; Seyed MohamadReza Hashemian; Hamidreza Jamaati; Majid Malekmohammad; Mona Gazi; Ian M. Adcock; Esmaeil Mortaz

    doi:10.21203/rs.3.rs-142927/v1 Date: 2021-01-07 Source: ResearchSquare

    A new coronavirus disease MESHD was described in December 2019 ( COVID-19 MESHD) in Wuhan City, Hubei Province, China and has reached pandemic status. According to the World Health Organization, the incubation time from being infected to symptom emergence averages 5-6 days for COVID-19 MESHD but can be up to 14 days. The mortality rate varies in different countries but is greater in elderly people and in patients with cardiovascular disease MESHD, diabetes MESHD and chronic respiratory diseases MESHD. Patients with chronic respiratory diseases MESHD often have reduced neutrophil function. We sought to measure neutrophil phagocytosis and bacterial killing in COVID-19 MESHD patients. 30 COVID-19 MESHD patients and 9 healthy individuals were recruited from the Masih Daneshvari Hospital (Tehran, Iran) from March-May 2020. Polymorphonuclear (PMN) cells were isolated from whole fresh blood and incubated with green fluorescent protein (GFP) labelled methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa. Phagocytosis was determined by measuring the florescence of co-cultures of bacteria and neutrophils and reported as the lag time before exponential growth. The number of viable bacteria was determined after 70 h by the Colony-Forming Unit (CFU). Bacterial phagocytosis of SA (22±0.9 versus 9.2±0.5h, p<0.01) and PA (12.4±0.6 versus 4.5±0.22, p<0.01) was significantly reduced in COVID-19 MESHD patients compared with healthy control subjects. After 70h there was a significant increase in CFU in COVID-19 MESHD subjects compared with healthy control subjects for both SA (2.6±0.09 x 108 versus 0.8±0.04 x 108 CFU/ml, p<0.001) and PA (2.2±0.09 x 109 versus 1.0±0.06 x 109 CFU/ml, p<0.001).These results suggests a defect in bacterial clearance by neutrophils in COVID-19 MESHD patients.  

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MeSH Disease
HGNC Genes
SARS-CoV-2 Proteins


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