Corpus overview


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MeSH Disease

Human Phenotype

Transmission

Seroprevalence
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    Evaluation of two commercial multiplex PCR tests for the diagnosis of acute respiratory infections MESHD in hospitalized children TRANS

    Authors: Le Wang; Shuo Yang; Xiaotong Yan; Teng Liu; Mengchuan Zhao; Dianping You; Guixia Li

    doi:10.21203/rs.3.rs-36565/v1 Date: 2020-06-19 Source: ResearchSquare

    Background Acute respiratory tract infections HP respiratory tract infections MESHD (ARTI), including the common cold, pharyngitis HP pharyngitis MESHD, sinusitis HP sinusitis MESHD, otitis media HP otitis media MESHD, tonsillitis HP tonsillitis MESHD, bronchiolitis HP bronchiolitis MESHD and pneumonia HP pneumonia MESHD are the most common diagnoses among pediatric patients and account for the majority of antibiotic prescriptions. A clear and rapid diagnosis is the key to preventing antibiotic abuse. Recently, based on different detection principles, many multi-target molecular analyses that can simultaneously detect dozens of pathogens have been developed, thereby greatly improving sensitivity SERO and shortening turnaround time. In this work, we conducted a head-to-head comparative study between melting curve analysis (MCA) and capillary electrophoresis assay (CE) in the detection of nine respiratory pathogens in sputum samples collected from hospitalized ARTI childre TRANS.Methods Through MCA and CE analysis, nine common respiratory pathogens were tested on hospitalized children TRANS under the age TRANS of 13 who met the ARTI criteria.Results A total of 237 children TRANS with sputum specimens were tested. For all the targets combined, the positive detection rate of XYRes-MCA was significantly higher than ResP-CE (72.2% vs. 63.7%, p = .002). Some pathogens were detected more often with MCA, such as parainfluenza virus, influenza B and coronavirus, and some pathogens do the opposite, such as adenovirus and influenza A (all p < .01). Very good kappa values for most of pathogens were observed, except for Influenza B and coronavirus (both κ = .39).Conclusions Multiplex melting curve and capillary electrophoresis assays performed similarly for the detection of common respiratory pathogens in hospitalized children TRANS, except for Influenza B and coronavirus. Higher sensitivity SERO was observed in the melting curve assay. By using this sensitive and rapid test SERO, it may improv patient prognosis and antimicrobial management.

    Evaluation of two commercial multiplex PCR tests for the diagnosis of acute respiratory infections MESHD in hospitalized children TRANS

    Authors: Le Wang; Shuo Yang; Xiaotong Yan; Teng Liu; Mengchuan Zhao; Zhishan Feng; Guixia Li

    doi:10.21203/rs.3.rs-20478/v1 Date: 2020-03-31 Source: ResearchSquare

    Background Acute respiratory tract infections HP respiratory tract infections MESHD (ARTI), including the common cold, pharyngitis HP pharyngitis MESHD, sinusitis HP sinusitis MESHD, otitis media HP otitis media MESHD, tonsillitis HP tonsillitis MESHD, bronchiolitis HP bronchiolitis MESHD and pneumonia HP pneumonia MESHD are the most common diagnoses in pediatric patients, and account for most antibiotic prescriptions. A confirmed and rapid ARTI diagnosis is key to preventing antibiotic abuse. Recently, based on different detection principles, many multi-target molecular analyses that can detect dozens of pathogens at the same time have been developed, greatly improving sensitivity SERO and shortening turnaround time. In this work, we performed a head-to-head comparative study between melting curve analysis (MCA) and capillary electrophoresis assay (CE) in the detection of nine respiratory pathogens in sputum samples collected from hospitalized children TRANS with ARTI. Methods By MCA and CE analysis, nine common respiratory pathogens were tested in hospitalized children TRANS< 13 years of age TRANS who met the ARTI criteria respectively. Results A total of 237 children TRANS with sputum specimens were tested. For all the targets combined, the positive detection rate of XYRes-MCA was significantly higher than that of ResP-CE (72.2% vs. 63.7%, p=.002). Some pathogens were detected more often with MCA, such as parainfluenza virus, influenza B and coronavirus, and some pathogens do the opposite, such as adenovirus and influenza A (all p<.01). Very good kappa values for most of pathogens were observed, except for Influenza B and coronavirus (both κ=.39). Conclusions Multiplex melting curve and capillary electrophoresis assays performed similarly for the detection of common respiratory pathogens in hospitalized children TRANS, except for Influenza B and coronavirus. A higher sensitivity SERO was observed in the melting curve assay. By using this sensitive and rapid test SERO, it may be possible to achieve improved patient prognosis and antimicrobial management.

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MeSH Disease
Human Phenotype
Transmission
Seroprevalence


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